Efficacy of opsonic and nonopsonic serotype 3 pneumococcal capsular polysaccharide-specific monoclonal antibodies against intranasal challenge with streptococcus pneumoniae in mice

Haijun Tian, Sarah Weber, Peter Thorkildson, Thomas R. Kozel, Liise-anne Pirofski

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Serotype-specific antibodies to pneumococcal capsular polysaccharide (PPS) are a critical component of vaccine-mediated immunity to Streptococcus pneumoniae. In this study, we investigated the in vitro opsonoph- agocytic activities of three PPS-specific mouse immunoglobulin G1 monoclonal antibodies (MAbs), 1E2, 5F6, and 7A9, and determined their in vivo efficacies against intranasal challenge with WU2, a serotype 3 pneumococcal strain, in normal and immunodeficient mice. The MAbs had different in vitro activities in a pneumococcal killing assay: 7A9 enhanced killing by mouse neutrophils and J774 cells in the presence of a complement source, whereas 5F6 promoted killing in the absence, but not the presence, of complement, and 1E2 did not promote killing under any conditions. Nonetheless, all three MAbs protected normal and complement component 3-deficient mice from a lethal intranasal challenge with WU2 in passive-immunization experiments in which 10 ng of the MAbs were administered intraperitoneally before intranasal challenge. In contrast, only 1E2 protected Fey receptor IIB knockout (FcγRIIB KO) mice and mice that were depleted of neutrophils with the MAb RB6, whereas 7A9 and 5F6 required neutrophils and FcγRIIB to mediate protection. Conversely, 7A9 and 5F6 protected FcγR KO mice, but 1E2 did not. Hence, the efficacy of 1E2 required an activating FcγR(s), whereas 5F6 and 7A9 required the inhibitory FcγR (FcγRIIB). Taken together, our data demonstrate that both MAbs that do and do not promote pneumococcal killing in vitro can mediate protection in vivo, although their efficacies depend on different host receptors and/or components.

Original languageEnglish (US)
Pages (from-to)1502-1513
Number of pages12
JournalInfection and Immunity
Volume77
Issue number4
DOIs
StatePublished - Apr 2009

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Streptococcus pneumoniae
Polysaccharides
Monoclonal Antibodies
Neutrophils
Complement C3
Passive Immunization
Knockout Mice
Serogroup
Immunoglobulins
Immunity
Vaccines
Antibodies
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Efficacy of opsonic and nonopsonic serotype 3 pneumococcal capsular polysaccharide-specific monoclonal antibodies against intranasal challenge with streptococcus pneumoniae in mice . / Tian, Haijun; Weber, Sarah; Thorkildson, Peter; Kozel, Thomas R.; Pirofski, Liise-anne.

In: Infection and Immunity, Vol. 77, No. 4, 04.2009, p. 1502-1513.

Research output: Contribution to journalArticle

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