Eficácia de uma vacina inativada, gE-deletada, contra o herpesvírus bovino tipo 1 (BoHV-1)

Translated title of the contribution: Efficacy of a gE-deleted, bovine herpesvirus 1 (BoHV- 1) inactivated vaccine

Alessandra D. Silva, Paulo A. Esteves, Diogenes Dezen, Anna P. Oliveira, Fernando R. Spilki, Fabrício S. Campos, Ana C. Franco, Paulo M. Roehe

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Bovine herpesvirus type 1 (BoHV-1) is recognized as a major cause of economic losses in cattle. Vaccination has been widely applied to minimize losses induced by BoHV-1 infections. We have previously reported the development of a differential BoHV-1 vaccine, based on a recombinant glycoprotein E (gE)-deleted virus (265gE-). In present paper the efficacy of such recombinant was evaluated as an inactivated vaccine. Five BoHV-1 seronegative calves were vaccinated intramuscularly on day 0 and boostered 30 days later with an inactivated, oil adjuvanted vaccine containing an antigenic mass equivalent to 107.0 fifty per cent cell culture infectious doses (CCID50) of 265gE-. Three calves were kept as non vaccinated controls. On day 60 post vaccination both vaccinated and controls were challenged with the virulent parental strain. No clinical signs or adverse effects were seen after or during vaccination. After challenge, 2/5 vaccinated calves showed mild clinical signs of infection, whereas all non vaccinated controls displayed intense rhinotracheitis and shed virus for longer and to higher titres than vaccinated calves. Serological responses were detected in all vaccinated animals after the second dose of vaccine, but not on control calves. Following corticosteroid administration in attempting to induce reactivation of the latent infection, no clinical signs were observed in vaccinated calves, whereas non vaccinated controls showed clinical signs of respiratory disease. In view of its immunogenicity and protective effect upon challenge with a virulent BoHV-1, the oil adjuvanted preparation with the inactivated 265gE- Erecombinant was shown to be suitable for use as a vaccine.

Translated title of the contributionEfficacy of a gE-deleted, bovine herpesvirus 1 (BoHV- 1) inactivated vaccine
Original languagePortuguese
Pages (from-to)545-551
Number of pages7
JournalPesquisa Veterinaria Brasileira
Volume29
Issue number7
DOIs
StatePublished - Jul 2009
Externally publishedYes

Keywords

  • BoHV-1
  • Bovine herpesvirus type 1
  • Inactivated vaccine
  • Recombinant vaccine

ASJC Scopus subject areas

  • veterinary(all)

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