Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves

W. Ray Waters; Mitchell V. Palmer; Brian J. Nonnecke; Tyler C. Thacker; Charles F.Capinos Scherer; D. Mark Estes; R. Glyn Hewinson; H. Martin Vordermeier; S. Whitney Barnes; Glenn C. Federe; John R. Walker; Richard J. Glynne; Tsungda Hsu; Brian Weinrick; Karolin Biermann; Michelle H. Larsen; William R. Jacobs

doi:10.1016/j.vaccine.2008.12.018

Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves

W. Ray Waters, Mitchell V. Palmer, Brian J. Nonnecke, Tyler C. Thacker, Charles F.Capinos Scherer, D. Mark Estes, R. Glyn Hewinson, H. Martin Vordermeier, S. Whitney Barnes, Glenn C. Federe, John R. Walker, Richard J. Glynne, Tsungda Hsu, Brian Weinrick, Karolin Biermann, Michelle H. Larsen, William R. Jacobs

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

An attenuated Mycobacterium bovis RD1 deletion (ΔRD1) mutant of the Ravenel strain was constructed, characterized, and sequenced. This M. bovis ΔRD1 vaccine strain administered to calves at 2 weeks of age provided similar efficacy as M. bovis bacillus Calmette Guerin (BCG) against low dose, aerosol challenge with virulent M. bovis at 3.5 months of age. Approximately 4.5 months after challenge, both ΔRD1- and BCG-vaccinates had reduced tuberculosis (TB)-associated pathology in lungs and lung-associated lymph nodes and M. bovis colonization of tracheobronchial lymph nodes as compared to non-vaccinates. Mean central memory responses elicited by either ΔRD1 or BCG prior to challenge correlated with reduced pathology and bacterial colonization. Neither ΔRD1 or BCG elicited IFN-γ responses to rESAT-6:CFP-10 prior to challenge, an emerging tool for modern TB surveillance programs. The ΔRD1 strain may prove useful for bovine TB vaccine programs, particularly if additional mutations are included to improve safety and immunogenicity.

Original language	English (US)
Pages (from-to)	1201-1209
Number of pages	9
Journal	Vaccine
Volume	27
Issue number	8
DOIs	https://doi.org/10.1016/j.vaccine.2008.12.018
State	Published - Feb 18 2009

Keywords

Aerosol inoculation
BCG
Live bacterial vaccine
Neonatal calf
RD1
Tuberculosis

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2008.12.018

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Waters, W. R., Palmer, M. V., Nonnecke, B. J., Thacker, T. C., Scherer, C. F. C., Estes, D. M., Hewinson, R. G., Vordermeier, H. M., Barnes, S. W., Federe, G. C., Walker, J. R., Glynne, R. J., Hsu, T., Weinrick, B., Biermann, K., Larsen, M. H., & Jacobs, W. R. (2009). Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves. Vaccine, 27(8), 1201-1209. https://doi.org/10.1016/j.vaccine.2008.12.018

Waters, WR, Palmer, MV, Nonnecke, BJ, Thacker, TC, Scherer, CFC, Estes, DM, Hewinson, RG, Vordermeier, HM, Barnes, SW, Federe, GC, Walker, JR, Glynne, RJ, Hsu, T, Weinrick, B, Biermann, K, Larsen, MH & Jacobs, WR 2009, 'Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves', Vaccine, vol. 27, no. 8, pp. 1201-1209. https://doi.org/10.1016/j.vaccine.2008.12.018

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title = "Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves",

abstract = "An attenuated Mycobacterium bovis RD1 deletion (ΔRD1) mutant of the Ravenel strain was constructed, characterized, and sequenced. This M. bovis ΔRD1 vaccine strain administered to calves at 2 weeks of age provided similar efficacy as M. bovis bacillus Calmette Guerin (BCG) against low dose, aerosol challenge with virulent M. bovis at 3.5 months of age. Approximately 4.5 months after challenge, both ΔRD1- and BCG-vaccinates had reduced tuberculosis (TB)-associated pathology in lungs and lung-associated lymph nodes and M. bovis colonization of tracheobronchial lymph nodes as compared to non-vaccinates. Mean central memory responses elicited by either ΔRD1 or BCG prior to challenge correlated with reduced pathology and bacterial colonization. Neither ΔRD1 or BCG elicited IFN-γ responses to rESAT-6:CFP-10 prior to challenge, an emerging tool for modern TB surveillance programs. The ΔRD1 strain may prove useful for bovine TB vaccine programs, particularly if additional mutations are included to improve safety and immunogenicity.",

keywords = "Aerosol inoculation, BCG, Live bacterial vaccine, Neonatal calf, RD1, Tuberculosis",

author = "Waters, {W. Ray} and Palmer, {Mitchell V.} and Nonnecke, {Brian J.} and Thacker, {Tyler C.} and Scherer, {Charles F.Capinos} and Estes, {D. Mark} and Hewinson, {R. Glyn} and Vordermeier, {H. Martin} and Barnes, {S. Whitney} and Federe, {Glenn C.} and Walker, {John R.} and Glynne, {Richard J.} and Tsungda Hsu and Brian Weinrick and Karolin Biermann and Larsen, {Michelle H.} and Jacobs, {William R.}",

note = "Funding Information: Work in the laboratory is supported in part by Grants AI 19042, CA 16858, CA 22736, and CA 13696 from the National Institutes of Health and by a grant from Becton Dickinson. S.-U.S. is supported by the Cancer Research Institute/J. M. Foundation Fellowship.",

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doi = "10.1016/j.vaccine.2008.12.018",

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T1 - Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves

AU - Waters, W. Ray

AU - Palmer, Mitchell V.

AU - Nonnecke, Brian J.

AU - Thacker, Tyler C.

AU - Scherer, Charles F.Capinos

AU - Estes, D. Mark

AU - Hewinson, R. Glyn

AU - Vordermeier, H. Martin

AU - Barnes, S. Whitney

AU - Federe, Glenn C.

AU - Walker, John R.

AU - Glynne, Richard J.

AU - Hsu, Tsungda

AU - Weinrick, Brian

AU - Biermann, Karolin

AU - Larsen, Michelle H.

AU - Jacobs, William R.

N1 - Funding Information: Work in the laboratory is supported in part by Grants AI 19042, CA 16858, CA 22736, and CA 13696 from the National Institutes of Health and by a grant from Becton Dickinson. S.-U.S. is supported by the Cancer Research Institute/J. M. Foundation Fellowship.

PY - 2009/2/18

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N2 - An attenuated Mycobacterium bovis RD1 deletion (ΔRD1) mutant of the Ravenel strain was constructed, characterized, and sequenced. This M. bovis ΔRD1 vaccine strain administered to calves at 2 weeks of age provided similar efficacy as M. bovis bacillus Calmette Guerin (BCG) against low dose, aerosol challenge with virulent M. bovis at 3.5 months of age. Approximately 4.5 months after challenge, both ΔRD1- and BCG-vaccinates had reduced tuberculosis (TB)-associated pathology in lungs and lung-associated lymph nodes and M. bovis colonization of tracheobronchial lymph nodes as compared to non-vaccinates. Mean central memory responses elicited by either ΔRD1 or BCG prior to challenge correlated with reduced pathology and bacterial colonization. Neither ΔRD1 or BCG elicited IFN-γ responses to rESAT-6:CFP-10 prior to challenge, an emerging tool for modern TB surveillance programs. The ΔRD1 strain may prove useful for bovine TB vaccine programs, particularly if additional mutations are included to improve safety and immunogenicity.

AB - An attenuated Mycobacterium bovis RD1 deletion (ΔRD1) mutant of the Ravenel strain was constructed, characterized, and sequenced. This M. bovis ΔRD1 vaccine strain administered to calves at 2 weeks of age provided similar efficacy as M. bovis bacillus Calmette Guerin (BCG) against low dose, aerosol challenge with virulent M. bovis at 3.5 months of age. Approximately 4.5 months after challenge, both ΔRD1- and BCG-vaccinates had reduced tuberculosis (TB)-associated pathology in lungs and lung-associated lymph nodes and M. bovis colonization of tracheobronchial lymph nodes as compared to non-vaccinates. Mean central memory responses elicited by either ΔRD1 or BCG prior to challenge correlated with reduced pathology and bacterial colonization. Neither ΔRD1 or BCG elicited IFN-γ responses to rESAT-6:CFP-10 prior to challenge, an emerging tool for modern TB surveillance programs. The ΔRD1 strain may prove useful for bovine TB vaccine programs, particularly if additional mutations are included to improve safety and immunogenicity.

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ER -

Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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