Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves

W. Ray Waters, Mitchell V. Palmer, Brian J. Nonnecke, Tyler C. Thacker, Charles F Capinos Scherer, D. Mark Estes, R. Glyn Hewinson, H. Martin Vordermeier, S. Whitney Barnes, Glenn C. Federe, John R. Walker, Richard J. Glynne, Tsungda Hsu, Brian Weinrick, Karolin Biermann, Michelle H. Larsen, William R. Jacobs

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

An attenuated Mycobacterium bovis RD1 deletion (ΔRD1) mutant of the Ravenel strain was constructed, characterized, and sequenced. This M. bovis ΔRD1 vaccine strain administered to calves at 2 weeks of age provided similar efficacy as M. bovis bacillus Calmette Guerin (BCG) against low dose, aerosol challenge with virulent M. bovis at 3.5 months of age. Approximately 4.5 months after challenge, both ΔRD1- and BCG-vaccinates had reduced tuberculosis (TB)-associated pathology in lungs and lung-associated lymph nodes and M. bovis colonization of tracheobronchial lymph nodes as compared to non-vaccinates. Mean central memory responses elicited by either ΔRD1 or BCG prior to challenge correlated with reduced pathology and bacterial colonization. Neither ΔRD1 or BCG elicited IFN-γ responses to rESAT-6:CFP-10 prior to challenge, an emerging tool for modern TB surveillance programs. The ΔRD1 strain may prove useful for bovine TB vaccine programs, particularly if additional mutations are included to improve safety and immunogenicity.

Original languageEnglish (US)
Pages (from-to)1201-1209
Number of pages9
JournalVaccine
Volume27
Issue number8
DOIs
StatePublished - Feb 18 2009

Fingerprint

Mycobacterium Infections
Mycobacterium bovis
Mycobacterium bovis BCG
aerosols
Aerosols
immune response
calves
infection
tuberculosis
lymph nodes
lungs
vaccines
bacterial colonization
bovine tuberculosis
Tuberculosis
Lymph Nodes
Tuberculosis Vaccines
Bovine Tuberculosis
Pathology
Lung

Keywords

  • Aerosol inoculation
  • BCG
  • Live bacterial vaccine
  • Neonatal calf
  • RD1
  • Tuberculosis

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Waters, W. R., Palmer, M. V., Nonnecke, B. J., Thacker, T. C., Scherer, C. F. C., Estes, D. M., ... Jacobs, W. R. (2009). Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves. Vaccine, 27(8), 1201-1209. https://doi.org/10.1016/j.vaccine.2008.12.018

Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves. / Waters, W. Ray; Palmer, Mitchell V.; Nonnecke, Brian J.; Thacker, Tyler C.; Scherer, Charles F Capinos; Estes, D. Mark; Hewinson, R. Glyn; Vordermeier, H. Martin; Barnes, S. Whitney; Federe, Glenn C.; Walker, John R.; Glynne, Richard J.; Hsu, Tsungda; Weinrick, Brian; Biermann, Karolin; Larsen, Michelle H.; Jacobs, William R.

In: Vaccine, Vol. 27, No. 8, 18.02.2009, p. 1201-1209.

Research output: Contribution to journalArticle

Waters, WR, Palmer, MV, Nonnecke, BJ, Thacker, TC, Scherer, CFC, Estes, DM, Hewinson, RG, Vordermeier, HM, Barnes, SW, Federe, GC, Walker, JR, Glynne, RJ, Hsu, T, Weinrick, B, Biermann, K, Larsen, MH & Jacobs, WR 2009, 'Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves', Vaccine, vol. 27, no. 8, pp. 1201-1209. https://doi.org/10.1016/j.vaccine.2008.12.018
Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Scherer CFC, Estes DM et al. Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves. Vaccine. 2009 Feb 18;27(8):1201-1209. https://doi.org/10.1016/j.vaccine.2008.12.018
Waters, W. Ray ; Palmer, Mitchell V. ; Nonnecke, Brian J. ; Thacker, Tyler C. ; Scherer, Charles F Capinos ; Estes, D. Mark ; Hewinson, R. Glyn ; Vordermeier, H. Martin ; Barnes, S. Whitney ; Federe, Glenn C. ; Walker, John R. ; Glynne, Richard J. ; Hsu, Tsungda ; Weinrick, Brian ; Biermann, Karolin ; Larsen, Michelle H. ; Jacobs, William R. / Efficacy and immunogenicity of Mycobacterium bovis ΔRD1 against aerosol M. bovis infection in neonatal calves. In: Vaccine. 2009 ; Vol. 27, No. 8. pp. 1201-1209.
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