Effects of umbilical cord wharton’s jelly mesenchymal stem cell transplantation on the expression of inflammatory factors in rats with spinal cord injury

Shan Shan Ma, Rui Na Qu, Yi Tian, Ning Yao, Yuan Bo Cui, Kang Han, Qu Xing, Bo Yang, Fangxia Guan

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The production and release of a large amount of inflammatory factors caused by immune system inflammatory response mainly contributes to secondary spinal cord injury. OBJECTIVE: To investigate the effects of umbilical cord Wharton’s jelly mesenchymal stem cell transplantation on repair of injured neurological function and expression of inflammatory factors monocyte chemoattractant protein 1 and interleukin 10 in rats with acute spinal cord injury. METHODS: Eighty-one healthy adult male Sprague-Dawley rats were randomly and equally divided into sham operation, model and cell transplantation groups, with 27 rats per group. Rats in the latter two groups were subjected to hemisection of the spinal cord to establish acute spinal cord injury models. Rat models i the cell transplantation group received umbilical cord Wharton’s jelly mesenchymal stem cell injection (1 ×106) 7a the tail vein. Rat neurological function was evaluated using the BBB score at different time points after spinal cord injury. The expression of monocyte chemoattractant protein 1 and interleukin 10 in injured spinal cord tissue was detected using ELISA assay at different time points after spinal cord injury. Migration and neuronal differentiation of umbilical cord Wharton’s jelly mesenchymal stem cells in the injured spinal cord tissue were determined using immunohistochemical staining method. RESULTS AND CONCLUSION: Compared with the sham operation and model groups, rat neurological function was significantly recovered in the cell transplantation group (P < 0.05). Compared to the model group, monocyte chemoattractant protein 1 level in the serum and monocyte chemoattractant protein 1 mRNA and protein expression in the injured spinal cord tissue were significantly lower (P < 0.05), but interleukin 10 mRNA and protein expression in the injured spinal cord tissue was significantly higher (P < 0.05), in the cell transplantation group. In the cell transplantation group, umbilical cord Wharton’s jelly mesenchymal stem cells could migrate to the injured region and express glinl fibrillary acidic protein. These tidings suggest that umbilical cord Wharon’s jelly mesenchymal stem cells promote rat neurological functinn recovery by regulating the inflammator response in the injured spinal cord tissue, which is likely to be one of mechanisms by which transplantation of umbilical cord Wharon’s jelly mesenchymal stem cells treats spinal cord injury.

Original languageEnglish (US)
Pages (from-to)3729-3735
Number of pages7
JournalChinese Journal of Tissue Engineering Research
Volume19
Issue number23
DOIs
StatePublished - Jun 4 2015
Externally publishedYes

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Wharton Jelly
Mesenchymal Stem Cell Transplantation
Umbilical Cord
Stem cells
Spinal Cord Injuries
Rats
Cell Transplantation
Spinal Cord
Mesenchymal Stromal Cells
Chemokine CCL2
Interleukin-10
Proteins
Tissue
Messenger RNA
Sprague Dawley Rats
Tail
Veins
Immune System
Transplantation
Enzyme-Linked Immunosorbent Assay

Keywords

  • Interleukin-10
  • Spinal Cord Injuries
  • Stem Cells Transplantation

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Clinical Biochemistry

Cite this

Effects of umbilical cord wharton’s jelly mesenchymal stem cell transplantation on the expression of inflammatory factors in rats with spinal cord injury. / Ma, Shan Shan; Qu, Rui Na; Tian, Yi; Yao, Ning; Cui, Yuan Bo; Han, Kang; Xing, Qu; Yang, Bo; Guan, Fangxia.

In: Chinese Journal of Tissue Engineering Research, Vol. 19, No. 23, 04.06.2015, p. 3729-3735.

Research output: Contribution to journalArticle

Ma, Shan Shan ; Qu, Rui Na ; Tian, Yi ; Yao, Ning ; Cui, Yuan Bo ; Han, Kang ; Xing, Qu ; Yang, Bo ; Guan, Fangxia. / Effects of umbilical cord wharton’s jelly mesenchymal stem cell transplantation on the expression of inflammatory factors in rats with spinal cord injury. In: Chinese Journal of Tissue Engineering Research. 2015 ; Vol. 19, No. 23. pp. 3729-3735.
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abstract = "BACKGROUND: The production and release of a large amount of inflammatory factors caused by immune system inflammatory response mainly contributes to secondary spinal cord injury. OBJECTIVE: To investigate the effects of umbilical cord Wharton’s jelly mesenchymal stem cell transplantation on repair of injured neurological function and expression of inflammatory factors monocyte chemoattractant protein 1 and interleukin 10 in rats with acute spinal cord injury. METHODS: Eighty-one healthy adult male Sprague-Dawley rats were randomly and equally divided into sham operation, model and cell transplantation groups, with 27 rats per group. Rats in the latter two groups were subjected to hemisection of the spinal cord to establish acute spinal cord injury models. Rat models i the cell transplantation group received umbilical cord Wharton’s jelly mesenchymal stem cell injection (1 ×106) 7a the tail vein. Rat neurological function was evaluated using the BBB score at different time points after spinal cord injury. The expression of monocyte chemoattractant protein 1 and interleukin 10 in injured spinal cord tissue was detected using ELISA assay at different time points after spinal cord injury. Migration and neuronal differentiation of umbilical cord Wharton’s jelly mesenchymal stem cells in the injured spinal cord tissue were determined using immunohistochemical staining method. RESULTS AND CONCLUSION: Compared with the sham operation and model groups, rat neurological function was significantly recovered in the cell transplantation group (P < 0.05). Compared to the model group, monocyte chemoattractant protein 1 level in the serum and monocyte chemoattractant protein 1 mRNA and protein expression in the injured spinal cord tissue were significantly lower (P < 0.05), but interleukin 10 mRNA and protein expression in the injured spinal cord tissue was significantly higher (P < 0.05), in the cell transplantation group. In the cell transplantation group, umbilical cord Wharton’s jelly mesenchymal stem cells could migrate to the injured region and express glinl fibrillary acidic protein. These tidings suggest that umbilical cord Wharon’s jelly mesenchymal stem cells promote rat neurological functinn recovery by regulating the inflammator response in the injured spinal cord tissue, which is likely to be one of mechanisms by which transplantation of umbilical cord Wharon’s jelly mesenchymal stem cells treats spinal cord injury.",
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AU - Cui, Yuan Bo

AU - Han, Kang

AU - Xing, Qu

AU - Yang, Bo

AU - Guan, Fangxia

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