Effects of starvation and refeeding on adult male rat diaphragm contractility, fatigue, and fiber types

David J. Prezant, D. E. Valentine, H. H. Kim, E. I. Gentry

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effects of 4.5 days of acute starvation, either alone or followed by refeeding (ad libitum), on diaphragm contractility, fatigue, and fiber types were studied in male rats. Contractility and fatigue resistance indexes were measured in an in vitro costal diaphragm strip preparation with direct stimulation at 37°C. Compared with controls, starvation produced a 28 ± 1% (P < 0.001) reduction in body weight and an 18 ± 4% (P < 0.001) reduction in costal diaphragm weight. Twitch and tetanic tensions (normalized for weight or cross-sectional area) were not reduced by starvation. Starvation produced significant increases in fatigue resistance indexes after a 5-Hz stimulation paradigm but not after a 100-Hz paradigm, supporting the hypothesis that fatigue resistance is dependent on the energy demand of a given paradigm. The proportions of type I and type II fibers were similar between diaphragms of starved and control rats, but the cross-sectional area of type II fibers decreased significantly by 18 ± 7% (P < 0.01). Thus, despite the significant decrease in diaphragm weight after starvation, contractility was preserved and fatigue resistance was increased (low-output paradigm). This is consistent with the decrease in type II fiber area. Refeeding restored all parameters so that there were no longer significant differences in body or diaphragm weight contractility, fatigue, or fiber types.

Original languageEnglish (US)
Pages (from-to)742-749
Number of pages8
JournalJournal of Applied Physiology
Volume74
Issue number2
StatePublished - 1993

Fingerprint

Starvation
Diaphragm
Fatigue
Weights and Measures
Body Weight

Keywords

  • respiratory muscles
  • undernutrition

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Effects of starvation and refeeding on adult male rat diaphragm contractility, fatigue, and fiber types. / Prezant, David J.; Valentine, D. E.; Kim, H. H.; Gentry, E. I.

In: Journal of Applied Physiology, Vol. 74, No. 2, 1993, p. 742-749.

Research output: Contribution to journalArticle

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