Effects of sex hormones on fluid and solute transport in Madin-Darby canine kidney cells

S. Sandhu, S. R. Silbiger, J. Lei, Joel Neugarten

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Polycystic kidney disease progresses more rapidly in men than in women. To investigate the basis for this sexual dimorphism, we exposed Madin-Darby canine kidney (MDCK) cells grown on collagen-coated cell culture inserts to control media, or to estradiol or testosterone (1 nM-1 μM). Compared to control and estradiol-treated cells, testosterone stimulated fluid secretion in a dose-dependent manner, enhancing fluid secretion 4.8-fold at 1 nM and 19.7-fold at 1 μM (0.59 ± 0.18 vs. 0.03 ± 0.01 μl/cm2/hr, P < 0.001). Chloride transport paralleled fluid secretion. Testosterone increased cellular cyclic AMP levels 3.2-fold at 1 nM and 12.3-fold at 1 μM (81.3 ± 30.7 vs. 6.6 ± 3.3 pmol/mg protein, P < 0.001). GDPβS (500 μM), an inhibitor of Gs, and 2',3'-dideoxyadenosine (10 μM), an inhibitor of the catalytic subunit of adenylate cyclase, suppressed testosterone-induced fluid and solute secretion. Neither testosterone nor estradiol had any effect on microsomal Na,K-ATPase activity, cellular proliferation or cellular total protein content. Our studies show that testosterone stimulates fluid secretion and solute transport by MDCK cells by increasing cAMP generation. In vivo, testosterone may contribute to cyst expansion by enhancing fluid secretion. This observation may help explain the worse prognosis of polycystic kidney disease observed in men.

Original languageEnglish (US)
Pages (from-to)1535-1539
Number of pages5
JournalKidney International
Volume51
Issue number5
StatePublished - 1997

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Fluids and Secretions
Madin Darby Canine Kidney Cells
Gonadal Steroid Hormones
Testosterone
Estradiol
Polycystic Kidney Diseases
Dideoxyadenosine
Adenylyl Cyclases
Sex Characteristics
Cyclic AMP
Cysts
Chlorides
Catalytic Domain
Proteins
Collagen
Cell Culture Techniques
Cell Proliferation

ASJC Scopus subject areas

  • Nephrology

Cite this

Effects of sex hormones on fluid and solute transport in Madin-Darby canine kidney cells. / Sandhu, S.; Silbiger, S. R.; Lei, J.; Neugarten, Joel.

In: Kidney International, Vol. 51, No. 5, 1997, p. 1535-1539.

Research output: Contribution to journalArticle

Sandhu, S. ; Silbiger, S. R. ; Lei, J. ; Neugarten, Joel. / Effects of sex hormones on fluid and solute transport in Madin-Darby canine kidney cells. In: Kidney International. 1997 ; Vol. 51, No. 5. pp. 1535-1539.
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