TY - JOUR
T1 - Effects of radiation type and delivery mode on a radioresistant eukaryote Cryptococcus neoformans
AU - Shuryak, Igor
AU - Bryan, Ruth A.
AU - Broitman, Jack
AU - Marino, Stephen A.
AU - Morgenstern, Alfred
AU - Apostolidis, Christos
AU - Dadachova, Ekaterina
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Introduction: Most research on radioresistant fungi, particularly on human pathogens such as Cryptococcus neoformans, involves sparsely-ionizing radiation. Consequently, fungal responses to densely-ionizing radiation, which can be harnessed to treat life-threatening fungal infections, remain incompletely understood. Methods: We addressed this issue by quantifying and comparing the effects of densely-ionizing α-particles (delivered either by external beam or by 213Bi-labeled monoclonal antibodies), and sparsely-ionizing 137Cs γ-rays, on Cryptococus neoformans. Results: The best-fit linear-quadratic parameters for clonogenic survival were the following: α=0.24×10-2Gy-1 for γ-rays and 1.07×10-2Gy-1 for external-beam α-particles, and β=1.44×10-5Gy-2 for both radiation types. Fungal cell killing by radiolabeled antibodies was consistent with predictions based on the α-particle dose to the cell nucleus and the linear-quadratic parameters for external-beam α-particles. The estimated RBE (for α-particles vs. γ-rays) at low doses was 4.47 for the initial portion of the α-particle track, and 7.66 for the Bragg peak. Non-radiological antibody effects accounted for up to 23% of cell death. Conclusions: These results quantify the degree of C. neoformans resistance to densely-ionizing radiations, and show how this resistance can be overcome with fungus-specific radiolabeled antibodies.
AB - Introduction: Most research on radioresistant fungi, particularly on human pathogens such as Cryptococcus neoformans, involves sparsely-ionizing radiation. Consequently, fungal responses to densely-ionizing radiation, which can be harnessed to treat life-threatening fungal infections, remain incompletely understood. Methods: We addressed this issue by quantifying and comparing the effects of densely-ionizing α-particles (delivered either by external beam or by 213Bi-labeled monoclonal antibodies), and sparsely-ionizing 137Cs γ-rays, on Cryptococus neoformans. Results: The best-fit linear-quadratic parameters for clonogenic survival were the following: α=0.24×10-2Gy-1 for γ-rays and 1.07×10-2Gy-1 for external-beam α-particles, and β=1.44×10-5Gy-2 for both radiation types. Fungal cell killing by radiolabeled antibodies was consistent with predictions based on the α-particle dose to the cell nucleus and the linear-quadratic parameters for external-beam α-particles. The estimated RBE (for α-particles vs. γ-rays) at low doses was 4.47 for the initial portion of the α-particle track, and 7.66 for the Bragg peak. Non-radiological antibody effects accounted for up to 23% of cell death. Conclusions: These results quantify the degree of C. neoformans resistance to densely-ionizing radiations, and show how this resistance can be overcome with fungus-specific radiolabeled antibodies.
KW - Alpha particles
KW - Cryptococcus neoformans
KW - Radiation
KW - Radiolabeled antibodies
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U2 - 10.1016/j.nucmedbio.2015.02.006
DO - 10.1016/j.nucmedbio.2015.02.006
M3 - Article
C2 - 25800676
AN - SCOPUS:84928827714
SN - 0969-8051
VL - 42
SP - 515
EP - 523
JO - Nuclear Medicine and Biology
JF - Nuclear Medicine and Biology
IS - 6
ER -