Effects of murine tumor necrosis factor on Friend erythroleukemic cells

Kimita Suyama, J. Goldstein, S. Green

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tumor necrosis inducing factor (TNF), a 140,000 molecular weight glycoprotein present in the serum of Corynebacterium parvum endotoxin-treated mice, was cytotoxic toward Friend virus-transformed erythroleukemic cells (FELC). These cells grow in culture as undifferentiated pro-erythroblasts but can be induced to differentiate in a limited fashion along the erythroid pathway to orthochromatic normoblasts by various agents such as dimethylsulfoxide (DMSO). Partially and highly purified preparations of TNF were cytotoxic toward logarithmically growing FELC whereas a comparable serum protein fraction from C. parvum treated mice or endotoxin from E. coli had no effect upon FELC viability. DMSO-induced cells were more sensitive to the action of TNF requiring only about half the concentration needed to produce 50% kill in noninduced cells. Inhibition of hemoglobin formation was TNF dose-related and could be decreased by 94%. TNF was also cytotoxic toward DMSO-induced cells in stationary phase and mitomycin C treated noninduced FELC. Neuraminidase modification of the surface of FELC increased the cytotoxicity of TNF by 50%. These results demonstrate that TNF destroys FELC whether they are nondividing, dividing or partially differentiated and suggest that TNF may accomplish this by affecting cell metabolism after internalization.

Original languageEnglish (US)
Pages (from-to)85-92
Number of pages8
JournalExperimental Cell Biology
Volume53
Issue number2
StatePublished - 1985
Externally publishedYes

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Friend murine leukemia virus
Tumor Necrosis Factor-alpha
Dimethyl Sulfoxide
Erythroblasts
Endotoxins
Propionibacterium acnes
Neuraminidase
Mitomycin
Protein C
Blood Proteins
Cell Survival
Glycoproteins
Hemoglobins
Molecular Weight
Escherichia coli
Serum

ASJC Scopus subject areas

  • Cell Biology

Cite this

Effects of murine tumor necrosis factor on Friend erythroleukemic cells. / Suyama, Kimita; Goldstein, J.; Green, S.

In: Experimental Cell Biology, Vol. 53, No. 2, 1985, p. 85-92.

Research output: Contribution to journalArticle

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