Effects of microcystin-LR exposure on spermiogenesis in nematode caenorhabditis elegans

Yunhui Li; Minhui Zhang; Pan Chen; Ran Liu; Geyu Liang; Lihong Yin; Yuepu Pu

doi:10.3390/ijms160922927

Effects of microcystin-LR exposure on spermiogenesis in nematode caenorhabditis elegans

Yunhui Li, Minhui Zhang, Pan Chen, Ran Liu, Geyu Liang, Lihong Yin, Yuepu Pu

Molecular Pharmacology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Little is known about the effect on spermiogenesis induced by microcystin-leucine arginine (MC-LR), even though such data are very important to better elucidate reproductive health. In the current work, with the aid of nematode Caenorhabditis elegans (C. elegans) as an animal model, we investigated the defects on spermiogenesis induced by MC-LR. Our results showed that MC-LR exposure induced sperm morphology abnormality and caused severe defects of sperm activation, trans-activation, sperm behavior and competition. Additionally, the expression levels of spe-15 were significantly decreased in C. elegans exposed to MC-LR lower than 16.0 μg/L, while the expression levels of spe-10 and fer-1 could be significantly lowered in C. elegans even exposed to 1.0 μg/L of MC-LR. Therefore, the present study reveals that MC-LR can induce adverse effects on spermiogenesis, and those defects of sperm functions may be induced by the decreases of spe-10, spe-15 and fer-1 gene expressions in C. elegans.

Original language	English (US)
Pages (from-to)	22927-22937
Number of pages	11
Journal	International Journal of Molecular Sciences
Volume	16
Issue number	9
DOIs	https://doi.org/10.3390/ijms160922927
State	Published - Sep 22 2015

Keywords

Caenorhabditis elegans
Microcystin-LR
Reproductive toxicity
Spermiogenesis

ASJC Scopus subject areas

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms160922927

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title = "Effects of microcystin-LR exposure on spermiogenesis in nematode caenorhabditis elegans",

abstract = "Little is known about the effect on spermiogenesis induced by microcystin-leucine arginine (MC-LR), even though such data are very important to better elucidate reproductive health. In the current work, with the aid of nematode Caenorhabditis elegans (C. elegans) as an animal model, we investigated the defects on spermiogenesis induced by MC-LR. Our results showed that MC-LR exposure induced sperm morphology abnormality and caused severe defects of sperm activation, trans-activation, sperm behavior and competition. Additionally, the expression levels of spe-15 were significantly decreased in C. elegans exposed to MC-LR lower than 16.0 μg/L, while the expression levels of spe-10 and fer-1 could be significantly lowered in C. elegans even exposed to 1.0 μg/L of MC-LR. Therefore, the present study reveals that MC-LR can induce adverse effects on spermiogenesis, and those defects of sperm functions may be induced by the decreases of spe-10, spe-15 and fer-1 gene expressions in C. elegans.",

keywords = "Caenorhabditis elegans, Microcystin-LR, Reproductive toxicity, Spermiogenesis",

author = "Yunhui Li and Minhui Zhang and Pan Chen and Ran Liu and Geyu Liang and Lihong Yin and Yuepu Pu",

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AU - Li, Yunhui

AU - Zhang, Minhui

AU - Chen, Pan

AU - Liu, Ran

AU - Liang, Geyu

AU - Yin, Lihong

AU - Pu, Yuepu

PY - 2015/9/22

Y1 - 2015/9/22

N2 - Little is known about the effect on spermiogenesis induced by microcystin-leucine arginine (MC-LR), even though such data are very important to better elucidate reproductive health. In the current work, with the aid of nematode Caenorhabditis elegans (C. elegans) as an animal model, we investigated the defects on spermiogenesis induced by MC-LR. Our results showed that MC-LR exposure induced sperm morphology abnormality and caused severe defects of sperm activation, trans-activation, sperm behavior and competition. Additionally, the expression levels of spe-15 were significantly decreased in C. elegans exposed to MC-LR lower than 16.0 μg/L, while the expression levels of spe-10 and fer-1 could be significantly lowered in C. elegans even exposed to 1.0 μg/L of MC-LR. Therefore, the present study reveals that MC-LR can induce adverse effects on spermiogenesis, and those defects of sperm functions may be induced by the decreases of spe-10, spe-15 and fer-1 gene expressions in C. elegans.

AB - Little is known about the effect on spermiogenesis induced by microcystin-leucine arginine (MC-LR), even though such data are very important to better elucidate reproductive health. In the current work, with the aid of nematode Caenorhabditis elegans (C. elegans) as an animal model, we investigated the defects on spermiogenesis induced by MC-LR. Our results showed that MC-LR exposure induced sperm morphology abnormality and caused severe defects of sperm activation, trans-activation, sperm behavior and competition. Additionally, the expression levels of spe-15 were significantly decreased in C. elegans exposed to MC-LR lower than 16.0 μg/L, while the expression levels of spe-10 and fer-1 could be significantly lowered in C. elegans even exposed to 1.0 μg/L of MC-LR. Therefore, the present study reveals that MC-LR can induce adverse effects on spermiogenesis, and those defects of sperm functions may be induced by the decreases of spe-10, spe-15 and fer-1 gene expressions in C. elegans.

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KW - Reproductive toxicity

KW - Spermiogenesis

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Effects of microcystin-LR exposure on spermiogenesis in nematode caenorhabditis elegans

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Keywords

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