Effects of microcystin-LR exposure on spermiogenesis in nematode caenorhabditis elegans

Yunhui Li, Minhui Zhang, Pan Chen, Ran Liu, Geyu Liang, Lihong Yin, Yuepu Pu

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Little is known about the effect on spermiogenesis induced by microcystin-leucine arginine (MC-LR), even though such data are very important to better elucidate reproductive health. In the current work, with the aid of nematode Caenorhabditis elegans (C. elegans) as an animal model, we investigated the defects on spermiogenesis induced by MC-LR. Our results showed that MC-LR exposure induced sperm morphology abnormality and caused severe defects of sperm activation, trans-activation, sperm behavior and competition. Additionally, the expression levels of spe-15 were significantly decreased in C. elegans exposed to MC-LR lower than 16.0 μg/L, while the expression levels of spe-10 and fer-1 could be significantly lowered in C. elegans even exposed to 1.0 μg/L of MC-LR. Therefore, the present study reveals that MC-LR can induce adverse effects on spermiogenesis, and those defects of sperm functions may be induced by the decreases of spe-10, spe-15 and fer-1 gene expressions in C. elegans.

Original languageEnglish (US)
Pages (from-to)22927-22937
Number of pages11
JournalInternational Journal of Molecular Sciences
Volume16
Issue number9
DOIs
Publication statusPublished - Sep 22 2015

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Keywords

  • Caenorhabditis elegans
  • Microcystin-LR
  • Reproductive toxicity
  • Spermiogenesis

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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