Effects of methylene blue photochemical virus inactivation on plasma components

BACKGROUND: Virus inactivation to blood is one of blood transfusion safety measures. The effect of virus inactivation on human plasma by methylene blue/light has been confirmed, but the report concerning virus inactivation to plasma on plasma components is very few. OBJECTIVE: To observe the effects of methylene blue photochemical virus inactivation to blood plasma on blood components' structure and function. METHODS: A total of 40 blood samples were randomly selected. Fresh plasma from 400 mL whole blood within 6 hours was prepared and the quality was weighed for reserve samples, and then the filter with methylene blue virus inactivation was sterilely connected. The final concentration of methylene blue was 0.9-1.3 μmol/L. Plasma added to methylene blue virus inactivation into 4 °C was put on a shelf box, with beat frequency of 60 times/minutes. 32 000-38 000 Lx intensity of 4 °C visible light was used to irradiate for 35 minutes. The residual methylene blue and white blood cells were filtered out from the plasma after illumination by the virus inactivated filters, mixed and remained sample 10 mL, and immediately placed in -80 °C refrigerator. The amount of plasma samples before and after exposure, methylene blue concentration, F VII: C, F V: C, VWF, Fib Content was detected. RESULTS AND CONCLUSION: After virus inactivated plasma, the recovery rate of plasma volume, F VII: C, F V: C, VWF, Fib were (96.39 ± 1.73)%, (82.55 ± 9.25)%, (81.03 ± 15.27)%, ( 93.25 ± 6.17)%, (81.61 ± 14.25)%. The effect of methylene blue photochemical virus inactivation in plasma on most components of the plasma is not obvious, and meets the clinical demand for safe blood transfusion.