Effects of H1 histones and a monoclonal autoantibody to H1 histones on clot formation in vitro: Possible implications in the antiphospholipid syndrome

Samir A. Kheiri, Thomas M. Fasy, Henny H. Billett

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Histones are known to bind anionic phospholipids (PLs). Binding of procoagulant PLs by histones released during cell injury/death may interfere with coagulation and may serve a local regulatory anticoagulant function. Histone H1 prolonged the PT and APTT of normal pooled plasma (NPP). These increased clotting times disappeared when anti-H1 monoclonal antibody (mAb) was added to the incubation. Dilute Russell Viper Venom Time was also prolonged with the addition of histone H1. When H1 was added to plasma from a patient with the antiphospholipid syndrome (APL plasma), there was a further prolongation of the abnormal APL clotting time which was partially corrected by anti-H1 mAb. Platelet neutralization times were increased with added H1 and were further increased using APL plasma. When disrupted endothelial cells were incubated with plasma with and without anti-H1 antibodies, the addition of anti-H1 antibodies decreased clotting times. These data support the theory that histones released during cell injury may have a regulatory anticoagulant role in clot formation and the anti-H1 effect of some APL plasmas may inhibit this, thereby contributing to thrombosis seen in APL patients.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalThrombosis Research
Volume82
Issue number1
DOIs
StatePublished - Apr 1 1996

Keywords

  • antiphospholipid antibodies
  • histones
  • lupus anticoagulant

ASJC Scopus subject areas

  • Hematology

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