Effects of H1 histones and a monoclonal autoantibody to H1 histones on clot formation in vitro

Possible implications in the antiphospholipid syndrome

Samir A. Kheiri, Thomas M. Fasy, Henny H. Billett

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Histones are known to bind anionic phospholipids (PLs). Binding of procoagulant PLs by histones released during cell injury/death may interfere with coagulation and may serve a local regulatory anticoagulant function. Histone H1 prolonged the PT and APTT of normal pooled plasma (NPP). These increased clotting times disappeared when anti-H1 monoclonal antibody (mAb) was added to the incubation. Dilute Russell Viper Venom Time was also prolonged with the addition of histone H1. When H1 was added to plasma from a patient with the antiphospholipid syndrome (APL plasma), there was a further prolongation of the abnormal APL clotting time which was partially corrected by anti-H1 mAb. Platelet neutralization times were increased with added H1 and were further increased using APL plasma. When disrupted endothelial cells were incubated with plasma with and without anti-H1 antibodies, the addition of anti-H1 antibodies decreased clotting times. These data support the theory that histones released during cell injury may have a regulatory anticoagulant role in clot formation and the anti-H1 effect of some APL plasmas may inhibit this, thereby contributing to thrombosis seen in APL patients.

Original languageEnglish (US)
Pages (from-to)43-50
Number of pages8
JournalThrombosis Research
Volume82
Issue number1
DOIs
StatePublished - Apr 1 1996

Fingerprint

Antiphospholipid Syndrome
Autoantibodies
Histones
Anticoagulants
Anti-Idiotypic Antibodies
Phospholipids
Monoclonal Antibodies
Prothrombin Time
Wounds and Injuries
In Vitro Techniques
Thrombosis
Cell Death
Blood Platelets
Endothelial Cells

Keywords

  • antiphospholipid antibodies
  • histones
  • lupus anticoagulant

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Effects of H1 histones and a monoclonal autoantibody to H1 histones on clot formation in vitro : Possible implications in the antiphospholipid syndrome. / Kheiri, Samir A.; Fasy, Thomas M.; Billett, Henny H.

In: Thrombosis Research, Vol. 82, No. 1, 01.04.1996, p. 43-50.

Research output: Contribution to journalArticle

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