Effects of desialylation of ovine submaxillary gland mucin (OSM) on humoral and cellular immune responses to Tn and sialylated Tn.

Kevin P. O'Boyle, Susan Coatsworth, Gillian Anthony, Mark Ramirez, Edward Greenwald, Ronald Kaleya, Jacob J. Steinberg, Janice P. Dutcher, Peter H. Wiernik

Research output: Contribution to journalArticle

Abstract

Resected carcinoma patients were immunized 3-5 times with ovine submaxillary gland mucin (OSM) containing predominantly sialylated Tn (sTn), completely desialylated ovine submaxillary gland mucin (dOSM) containing predominantly Tn, or 50% desialylated OSM containing Tn and sTn plus bacillus Calmette-Guerin (BCG) as an immunologic adjuvant. Pre- and postimmunization sera were quantified by ELISA, whole-cell ELISA, and immune stain dot blots. Fifteen of 17 patients produced IgG antibody titers from 40 to 5120 times more reactive with OSM and dOSM postimmunization. More importantly, these IgG antibodies reacted with LS-174T, a human colon carcinoma cell line. Significant DTH-like responses (1-17 cm) were observed in 15 of 17 patients; the strength of these responses was dependent on the presence or absence of sialic acid. Biopsies of these DTH-like reactions revealed infiltration with some CD8+ lymphocytes and mast cells. These results suggest that a single 9-carbon sugar can affect cellular immune responses to mucin antigens. It is thought that these large erythematous, nonindurated cellular reactions are antibody-mediated Arthus-like reactions. OSM, and especially dOSM, were also found to inhibit lymphocyte proliferation.

Original languageEnglish (US)
Pages (from-to)5
Number of pages1
JournalCancer immunity : a journal of the Academy of Cancer Immunology
Volume6
StatePublished - 2006
Externally publishedYes

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Submandibular Gland
Mucins
Humoral Immunity
Cellular Immunity
Sheep
Antibodies
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Arthus Reaction
Lymphocytes
Carcinoma
Immunologic Adjuvants
N-Acetylneuraminic Acid
Mycobacterium bovis
Mast Cells
Colon
Coloring Agents
Carbon
Biopsy
Antigens

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Effects of desialylation of ovine submaxillary gland mucin (OSM) on humoral and cellular immune responses to Tn and sialylated Tn. / O'Boyle, Kevin P.; Coatsworth, Susan; Anthony, Gillian; Ramirez, Mark; Greenwald, Edward; Kaleya, Ronald; Steinberg, Jacob J.; Dutcher, Janice P.; Wiernik, Peter H.

In: Cancer immunity : a journal of the Academy of Cancer Immunology, Vol. 6, 2006, p. 5.

Research output: Contribution to journalArticle

O'Boyle, Kevin P. ; Coatsworth, Susan ; Anthony, Gillian ; Ramirez, Mark ; Greenwald, Edward ; Kaleya, Ronald ; Steinberg, Jacob J. ; Dutcher, Janice P. ; Wiernik, Peter H. / Effects of desialylation of ovine submaxillary gland mucin (OSM) on humoral and cellular immune responses to Tn and sialylated Tn. In: Cancer immunity : a journal of the Academy of Cancer Immunology. 2006 ; Vol. 6. pp. 5.
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abstract = "Resected carcinoma patients were immunized 3-5 times with ovine submaxillary gland mucin (OSM) containing predominantly sialylated Tn (sTn), completely desialylated ovine submaxillary gland mucin (dOSM) containing predominantly Tn, or 50{\%} desialylated OSM containing Tn and sTn plus bacillus Calmette-Guerin (BCG) as an immunologic adjuvant. Pre- and postimmunization sera were quantified by ELISA, whole-cell ELISA, and immune stain dot blots. Fifteen of 17 patients produced IgG antibody titers from 40 to 5120 times more reactive with OSM and dOSM postimmunization. More importantly, these IgG antibodies reacted with LS-174T, a human colon carcinoma cell line. Significant DTH-like responses (1-17 cm) were observed in 15 of 17 patients; the strength of these responses was dependent on the presence or absence of sialic acid. Biopsies of these DTH-like reactions revealed infiltration with some CD8+ lymphocytes and mast cells. These results suggest that a single 9-carbon sugar can affect cellular immune responses to mucin antigens. It is thought that these large erythematous, nonindurated cellular reactions are antibody-mediated Arthus-like reactions. OSM, and especially dOSM, were also found to inhibit lymphocyte proliferation.",
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AU - Anthony, Gillian

AU - Ramirez, Mark

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AU - Kaleya, Ronald

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AU - Dutcher, Janice P.

AU - Wiernik, Peter H.

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