Effects of deoxycoformycin in mice. I.Suppression and enhancement of in vivo antibody responses to thymus-dependent and -independent antigens

H. Ratech, M. K. Bell, R. Hirschhorn, G. J. Thorbecke

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Abstract

The effect of 2'-deoxycoformycin (DCF) on the PFC responses of AKR mice to SE, TNP-Ficoll, and TNP-B. abortus was examined. Subcutaneous injection of DCF 4 days before antigen caused suppression of all three responses by 70 to 78%. In contrast, injection of DCF 1 day after antigen caused enhancement of both the anti-SE and the anti-TNP-Ficoll responses. Although a single high dose of cortisone acetate injected 4 days before antigen caused a similar suppression, the effect of DCF was not mediated via a steroid release, inasmuch as DCF also suppressed the immune response in adrenalectomized mice. The response of BALB/c mice to TNP-Ficoll was also inhibited by DCF pretreatment and enhanced by injection of DCF after antigen. In contrast, in athymic mice DCF caused suppression of the anti-TNP-Ficoll PFC response, whether injected before or after antigen. These results are interpreted as suggesting that DCF causes suppression primarily via an effect on B cells. The enhancement seen in normal but not in athymic mice may possibly be ascribed to an effect on suppressor T cells. Apparently the enhancement of both TFD and TI responses caused by DCF injected 1 day after antigen in normal mice is the net result of these two opposing effects. The results imply that helper T cells are resistant to DCF.

Original languageEnglish (US)
Pages (from-to)3071-3076
Number of pages6
JournalJournal of Immunology
Volume132
Issue number6
StatePublished - 1984
Externally publishedYes

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Pentostatin
T Independent Antigens
Antibody Formation
Antigens
Nude Mice
Inbred AKR Mouse
Injections
Subcutaneous Injections
Helper-Inducer T-Lymphocytes
B-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Effects of deoxycoformycin in mice. I.Suppression and enhancement of in vivo antibody responses to thymus-dependent and -independent antigens. / Ratech, H.; Bell, M. K.; Hirschhorn, R.; Thorbecke, G. J.

In: Journal of Immunology, Vol. 132, No. 6, 1984, p. 3071-3076.

Research output: Contribution to journalArticle

Ratech, H. ; Bell, M. K. ; Hirschhorn, R. ; Thorbecke, G. J. / Effects of deoxycoformycin in mice. I.Suppression and enhancement of in vivo antibody responses to thymus-dependent and -independent antigens. In: Journal of Immunology. 1984 ; Vol. 132, No. 6. pp. 3071-3076.
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