Effects of dantrolene on cooling times and cardiovascular parameters in an immature porcine model of heatstroke

Gary B. Zuckerman, Lewis P. Singer, David H. Rubin, Edward E. Conway

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: To evaluate the effects of dantrolene on cooling times and cardiovascular parameters in an immature porcine model of heatstroke. Design: Prospective, randomized, controlled, multigroup study. Setting: Research animal laboratory. Subjects: Yorkshire piglets (n = 16), 4 to 5 wks of age, 3.5 to 4.5 kg of body weight. Interventions: Animals were slowly heated with a radiant heat source to 43°C and then maintained at this temperature for 30 mins. The animals were then removed from the heat source and randomized into one of four groups to receive either conventional cooling methods consisting of fluid resuscitation with 0.9% sodium chloride solution, sponging with room temperature water, mechanical fanning, and gastric lavage with iced 0.9% sodium chloride solution (group 1), conventional cooling methods and dantrolene (group 2), conventional cooling methods and dantrolene's vehicle mannitol (group 3), or no treatment (group 4). Cooling times, defined as the time required to reach a core body temperature of 38.5°C, and cardiovascular parameters for each group were then compared. Measurements and Main Results: Animals in groups 1, 2, and 3 had significantly (p < .05) lower core body temperatures than animals that received no treatment at the conclusion of the experiment. Piglets in groups 2 and 3 had faster cooling times than piglets in group 1 (p < .05). However, there was no statistically significant difference in cooling times between the animals in groups 2 and 3. There were no statistically significant differences in heart rate, mean arterial pressure, central venous pressure, pulmonary artery occlusion pressure, or systemic vascular resistance index between animals in groups 1, 2, or 3. Group 3 piglets had higher cardiac indices and stroke indices than the piglets in the other groups (p < .05). Conclusions: Therapeutic interventions with conventional cooling or conventional cooling and dantrolene provided significant improvement in cardiovascular function in an immature porcine heatstroke model. Dantrolene, given with conventional cooling methods, offered no significant improvement in cardiovascular parameters compared with conventional cooling methods alone. Dantrolene significantly shortened the cooling time compared with conventional cooling but did not significantly shorten the cooling time compared with its vehicle, mannitol. Although dantrolene significantly shortened the cooling time, it did not appear to be superior compared with conventional cooling methods in treating heatstroke in this immature porcine heatstroke model.

Original languageEnglish (US)
Pages (from-to)135-139
Number of pages5
JournalCritical Care Medicine
Volume25
Issue number1
DOIs
StatePublished - Jan 1997

Fingerprint

Heat Stroke
Dantrolene
Swine
Mannitol
Body Temperature
Sodium Chloride
Hot Temperature
Gastric Lavage
Central Venous Pressure
Temperature
Laboratory Animals
Resuscitation
Vascular Resistance
Pulmonary Artery
Arterial Pressure
Therapeutics
Heart Rate
Stroke
Body Weight
Pressure

Keywords

  • cardiovascular
  • cooling times
  • dantrolene
  • heatstroke
  • hyperpyrexia
  • malignant hyperthermia
  • pediatric
  • porcine

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Effects of dantrolene on cooling times and cardiovascular parameters in an immature porcine model of heatstroke. / Zuckerman, Gary B.; Singer, Lewis P.; Rubin, David H.; Conway, Edward E.

In: Critical Care Medicine, Vol. 25, No. 1, 01.1997, p. 135-139.

Research output: Contribution to journalArticle

Zuckerman, Gary B. ; Singer, Lewis P. ; Rubin, David H. ; Conway, Edward E. / Effects of dantrolene on cooling times and cardiovascular parameters in an immature porcine model of heatstroke. In: Critical Care Medicine. 1997 ; Vol. 25, No. 1. pp. 135-139.
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N2 - Objective: To evaluate the effects of dantrolene on cooling times and cardiovascular parameters in an immature porcine model of heatstroke. Design: Prospective, randomized, controlled, multigroup study. Setting: Research animal laboratory. Subjects: Yorkshire piglets (n = 16), 4 to 5 wks of age, 3.5 to 4.5 kg of body weight. Interventions: Animals were slowly heated with a radiant heat source to 43°C and then maintained at this temperature for 30 mins. The animals were then removed from the heat source and randomized into one of four groups to receive either conventional cooling methods consisting of fluid resuscitation with 0.9% sodium chloride solution, sponging with room temperature water, mechanical fanning, and gastric lavage with iced 0.9% sodium chloride solution (group 1), conventional cooling methods and dantrolene (group 2), conventional cooling methods and dantrolene's vehicle mannitol (group 3), or no treatment (group 4). Cooling times, defined as the time required to reach a core body temperature of 38.5°C, and cardiovascular parameters for each group were then compared. Measurements and Main Results: Animals in groups 1, 2, and 3 had significantly (p < .05) lower core body temperatures than animals that received no treatment at the conclusion of the experiment. Piglets in groups 2 and 3 had faster cooling times than piglets in group 1 (p < .05). However, there was no statistically significant difference in cooling times between the animals in groups 2 and 3. There were no statistically significant differences in heart rate, mean arterial pressure, central venous pressure, pulmonary artery occlusion pressure, or systemic vascular resistance index between animals in groups 1, 2, or 3. Group 3 piglets had higher cardiac indices and stroke indices than the piglets in the other groups (p < .05). Conclusions: Therapeutic interventions with conventional cooling or conventional cooling and dantrolene provided significant improvement in cardiovascular function in an immature porcine heatstroke model. Dantrolene, given with conventional cooling methods, offered no significant improvement in cardiovascular parameters compared with conventional cooling methods alone. Dantrolene significantly shortened the cooling time compared with conventional cooling but did not significantly shorten the cooling time compared with its vehicle, mannitol. Although dantrolene significantly shortened the cooling time, it did not appear to be superior compared with conventional cooling methods in treating heatstroke in this immature porcine heatstroke model.

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