Effects of cytokines on synthesis and function of the hepatic asialoglycoprotein receptor

Ulrich Treichel, Elisabeth M. Paietta, Thomas Poralla, Karl Hermann Meyer Zum Büschenfelde, Richard J. Stockert

Research output: Contribution to journalArticle

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Abstract

In this study we have investigated whether cytokines, critical mediators of the immune response, might have a direct effect on the expression and/or function of the human hepatic asialoglycoprotein receptor (ASGPR). Binding and uptake of asialoglycoproteins by the human hepatoma cell line, HepG2, and by freshly isolated rat hepatocytes were inhibited by 50% after 3-6 hours and completely abolished following a 24 hour exposure to tumor necrosis factor (TNF) α, interferon (INF) α or γ, or interleukin-2 (IL-2). The loss of ASGPR binding activity mediated by IL-2 was reversible up to 4 hours of exposure and accompanied by the selective phosphorylation of the cell-surface receptor. Steady-state levels of total cellular ASGPR protein remained unchanged over the first 6 hours of IL-2 incubation but declined in a dose dependent manner thereafter. This down regulation of ASGPR expression was due to reduced synthesis as a result of reduced receptor transcript levels. No loss was detected, however, of cell surface-associated receptor protein even after 24 hours of IL-2 incubation, suggesting that cytokine induced phosphorylation constitutes a mechanism to regulate receptor activity.

Original languageEnglish (US)
Pages (from-to)527-534
Number of pages8
JournalJournal of Cellular Physiology
Volume158
Issue number3
StatePublished - Mar 1994

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Asialoglycoprotein Receptor
Interleukin-2
Cytokines
Phosphorylation
Cell Surface Receptors
Asialoglycoproteins
Interferons
Rats
Hepatocytes
Hepatocellular Carcinoma
Proteins
Down-Regulation
Tumor Necrosis Factor-alpha
Cells
Cell Line

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Treichel, U., Paietta, E. M., Poralla, T., Meyer Zum Büschenfelde, K. H., & Stockert, R. J. (1994). Effects of cytokines on synthesis and function of the hepatic asialoglycoprotein receptor. Journal of Cellular Physiology, 158(3), 527-534.

Effects of cytokines on synthesis and function of the hepatic asialoglycoprotein receptor. / Treichel, Ulrich; Paietta, Elisabeth M.; Poralla, Thomas; Meyer Zum Büschenfelde, Karl Hermann; Stockert, Richard J.

In: Journal of Cellular Physiology, Vol. 158, No. 3, 03.1994, p. 527-534.

Research output: Contribution to journalArticle

Treichel, U, Paietta, EM, Poralla, T, Meyer Zum Büschenfelde, KH & Stockert, RJ 1994, 'Effects of cytokines on synthesis and function of the hepatic asialoglycoprotein receptor', Journal of Cellular Physiology, vol. 158, no. 3, pp. 527-534.
Treichel U, Paietta EM, Poralla T, Meyer Zum Büschenfelde KH, Stockert RJ. Effects of cytokines on synthesis and function of the hepatic asialoglycoprotein receptor. Journal of Cellular Physiology. 1994 Mar;158(3):527-534.
Treichel, Ulrich ; Paietta, Elisabeth M. ; Poralla, Thomas ; Meyer Zum Büschenfelde, Karl Hermann ; Stockert, Richard J. / Effects of cytokines on synthesis and function of the hepatic asialoglycoprotein receptor. In: Journal of Cellular Physiology. 1994 ; Vol. 158, No. 3. pp. 527-534.
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