Effects of crystalloid, blood, and University of Wisconsin perfusates on weight, water content, and left ventricular compliance in an edema-prone, isolated porcine heart model

Z. C. Weng, A. C. Nicolosi, P. W. Detwiler, Daphne T. Hsu, S. W. Schierman, A. H. Goldstein, H. M. Spotnitz

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Coronary perfusion with blood and cardioplegic solutions was examined in isolated, arrested, hypothermic porcine hearts. Myocardial water content, heart weight, and left ventricular diastolic pressure-volume curves were measured before and after coronary perfusion. Statistics were based on exponential curve fitting to pressure-volume data and analysis of variance. Thirty-two pig hearts were divided into five experimental groups and a control group; after control measurements, each experimental group underwent three successive coronary perfusions with 1 L of unmodified blood or a solution of controlled osmolarity, 150 mOsm/L (diluted Plegisol solution), 280 mOsm/L (Plegisol solution and albumin), 334 mOsm/L (University of Wisconsin solution), or 380 mOsm/L (Stanford solution). After each perfusion, measurements were repeated. All experiments were completed within 90 minutes. The first perfusion was delayed 20 minutes after excision of the heart to allow for instrumentation. Each experimental group demonstrated a statistically significant increase in heart weight and myocardial water content and a significant decrease in left ventricular compliance after perfusion. Changes were less pronounced with blood than crystalloids. Edema effects were minimized but not prevented by hyperosmolarity. University of Wisconsin solution appeared unique in minimizing progressive edema after the first perfusion. Over the 81 perfusions studied, changes in left ventricular compliance were linearly related to heart weight and water content. We conclude that in this model, in which edema sensitivity is increased by delayed perfusion and venous occlusion, edema is minimized but not eliminated by whole blood and University of Wisconsin solution. The model appears useful in assessing properties of cardioplegia vehicles intended for use in the injured myocardium.

Original languageEnglish (US)
Pages (from-to)504-513
Number of pages10
JournalJournal of Thoracic and Cardiovascular Surgery
Volume103
Issue number3
StatePublished - 1992
Externally publishedYes

Fingerprint

Compliance
Edema
Swine
Perfusion
Weights and Measures
Water
Cardioplegic Solutions
crystalloid solutions
Induced Heart Arrest
Ventricular Pressure
Osmolar Concentration
Albumins
Myocardium
Analysis of Variance
Blood Pressure
Pressure
Control Groups

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Effects of crystalloid, blood, and University of Wisconsin perfusates on weight, water content, and left ventricular compliance in an edema-prone, isolated porcine heart model. / Weng, Z. C.; Nicolosi, A. C.; Detwiler, P. W.; Hsu, Daphne T.; Schierman, S. W.; Goldstein, A. H.; Spotnitz, H. M.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 103, No. 3, 1992, p. 504-513.

Research output: Contribution to journalArticle

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