Effects of camptothecin on the breakage and repair of DNA during the cell cyle

Susan Band Horwitz, M. S. Horwitz

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Incubation of camptothecin with HeLa cells in either the G1 or S phase of the cell cycle results in fragmentation of DNA as analyzed on alkaline sucrose gradients. Removal of the drug from G1 or S phase cells results in the normal sedimentation of cellular DNA. Neither the fragmentation nor the repair of HeLa cell DNA requires DNA replication. However, subtle irreversible changes must take place in the DNA in cells treated with camptothecin for 30 min during the S phase, since such cells are unable to undergo subsequent cell division. The lethal effect is presumed to be at the single strand replication forks.

Original languageEnglish (US)
Pages (from-to)2834-2836
Number of pages3
JournalCancer Research
Volume33
Issue number11
StatePublished - 1973

Fingerprint

Camptothecin
S Phase
DNA Repair
G1 Phase
HeLa Cells
DNA
DNA Fragmentation
DNA Replication
Cell Division
Sucrose
Cell Cycle
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effects of camptothecin on the breakage and repair of DNA during the cell cyle. / Band Horwitz, Susan; Horwitz, M. S.

In: Cancer Research, Vol. 33, No. 11, 1973, p. 2834-2836.

Research output: Contribution to journalArticle

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