Effects of calcium on recombinant bovine chromogranin A

Ruth Hogue Angeletti, Ghazala Ali, Ning Shen, Patricia Gee, Edward Nieves

Research output: Contribution to journalArticle

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Abstract

Bovine chromogranin A, the acidic calcium‐binding protein characteristic of endocrine secretory vesicles, has been expressed in Escherichia coli using the pET3a vector system under T7 polymerase control. The expressed protein is located in the bacterial cytosol and can be purified from bacterial proteins by a heat treatment step, followed by gel filtration, anion‐exchange, and reversed‐phase chromatography. The purified recombinant chromogranin A has an apparent MT of ca. 72,000 by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, in spite of its 432‐amino acid polypeptide chain, consistent with observations on natural chromogranin A. The primary structure has been confirmed by mass spectral analysis of tryptic peptides, by Edman degradation of the intact protein, and by immunoreactivity with sequence‐specific antibodies. Analysis by circular dichroism spectroscopy shows pH‐ and concentration‐dependent spectra. The spectra are Ca2+‐dependent from 5 to 40 μM.

Original languageEnglish (US)
Pages (from-to)1604-1612
Number of pages9
JournalProtein Science
Volume1
Issue number12
DOIs
Publication statusPublished - Dec 1992

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Keywords

  • calcium binding
  • chromogranin
  • circular dichroism
  • recombinant protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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