Effects of ADMA on gene expression and metabolism in serum-starved LoVo cells

Ningning Zheng, Ke Wang, Jiaojiao He, Yunping Qiu, Guoxiang Xie, Mingming Su, Wei Jia, Houkai Li

Research output: Contribution to journalArticle

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Abstract

Serum starvation is a typical way for inducing tumor cell apoptosis and stress. Asymmetric dimethylarginine (ADMA) is an endogenous metabolite. Our previous study reveals the plasma ADMA level is elevated in colon cancer patients, which can attenuate serum starvation-induced apoptosis in LoVo cells. In current study, we evaluated the effects of ADMA on gene expression and metabolism in serum-starved LoVo cells with gene microarray and metabolomic approaches. Our results indicated that 96 h serum starvation induced comprehensive alterations at transcriptional level, and most of them were restored by ADMA. The main signaling pathways induced by serum starvation included cancers-related pathways, pathways in cell death, apoptosis, and cell cycle etc. Meanwhile, the metabolomic data showed serum-starved cells were clearly separated with control cells, but not with ADMA-treated cells in PCA model. The identified differential metabolites indicated serum starvation significantly suppressed TCA cycle, altered glucose and fatty acids metabolism, as well as nucleic acids metabolism. However, very few differential metabolites were identified between ADMA and serum-starved cells. In summary, our current results indicated serum starvation profoundly altered the gene expression and metabolism of LoVo cells, whereas ADMA could restore most of the changes at transcriptional level, but not at metabolic level.

Original languageEnglish (US)
JournalScientific Reports
Volume6
DOIs
StatePublished - May 16 2016

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Starvation
Gene Expression
Serum
Metabolomics
Apoptosis
N,N-dimethylarginine
Passive Cutaneous Anaphylaxis
Colonic Neoplasms
Nucleic Acids
Neoplasms
Cell Cycle
Cell Death
Fatty Acids
Glucose
Genes

ASJC Scopus subject areas

  • General

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Effects of ADMA on gene expression and metabolism in serum-starved LoVo cells. / Zheng, Ningning; Wang, Ke; He, Jiaojiao; Qiu, Yunping; Xie, Guoxiang; Su, Mingming; Jia, Wei; Li, Houkai.

In: Scientific Reports, Vol. 6, 16.05.2016.

Research output: Contribution to journalArticle

Zheng, Ningning ; Wang, Ke ; He, Jiaojiao ; Qiu, Yunping ; Xie, Guoxiang ; Su, Mingming ; Jia, Wei ; Li, Houkai. / Effects of ADMA on gene expression and metabolism in serum-starved LoVo cells. In: Scientific Reports. 2016 ; Vol. 6.
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