Effective control of yellow stem borer and rice leaf folder in transgenic rice indica varieties Basmati 370 and M 7 using the novel δ-endotoxin cry2A Bacillus thuringiensis gene

Shahina Bano Maqbool, T. Husnain, S. Riazuddin, L. Masson, P. Christou

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

Transgenic rice indica varieties Basmati 370 and M 7 expressing the novel cry2A (Bt) insecticidal gene were generated by particle bombardment. Molecular and biochemical analyses in R0 and R1 populations confirmed stable integration and expression of this novel Bt transgene. We estimated that the gene product was expressed up to 5% of total leaf protein. Insect feeding bioassays demonstrated that the Cry2A protein was effective against the yellow stem borer and the rice leaf folder, two major rice pests in the Indian Subcontinent. This is the first report of the control of major rice pests using this specific Bt gene. The cry2A gene can now be used in combination with other insecticidal genes for pyramiding resistance against insect pests. This will delay, or perhaps in combination with integrated pest management practices, prevent evolution of insect populations resistant to single insecticidal genes.

Original languageEnglish (US)
Pages (from-to)501-507
Number of pages7
JournalMolecular Breeding
Volume4
Issue number6
DOIs
StatePublished - Dec 1 1998
Externally publishedYes

Keywords

  • Bacillus thuringiensis (Bt)
  • Cry2A
  • Particle bombardment
  • Rice leaf folder
  • Transgenic rice
  • Yellow stem borer

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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