Effect of voluntary exercise on genetically obese Cpe fat/fat mice

Quantitative proteomics of serum

Quan Yuan, Jose D. Fontenele-Neto, Lloyd D. Fricker

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: To compare the effect of voluntary exercise on body weight, food consumption, and levels of serum proteins between wild-type and carboxypeptidase E-deficient (Cpe fat/fat) mice. Research Methods and Procedures: Study 1 consisted of three groups of female mice: Cpe fat/fat mice with continuous access to exercise wheels for 3 weeks (n = 4); wild-type C57BKS mice with access to exercise wheels for 3 weeks (n = 4); and sedentary Cpe fat/fat mice (n = 3). Activity, body weight, and food consumption were monitored for this period and a subsequent 9-week period without exercise wheels. Study 2 consisted of four groups of male mice (n = 6 to 7 each): Cpe fat/fat mice with exercise wheels, wild-type mice with exercise wheels, and Cpe fat/fat and wild-type mice without exercise wheels. Body weight and food consumption were measured over 4 weeks. Sera were collected, and the protein profile was determined by 2-dimensional gel electrophoresis and mass spectrometry. Results: Cpe fat/fat mice were moderately hyperphagic but lost weight during the initial exercise period because of greater energy expenditure. The effect of exercise was temporary, and the mice gained weight after the second week. Several serum proteins were found to be altered by exercise: haptoglobin was decreased by exercise in Cpe fat/fat mice, and several kallikreins were increased by exercise in wild-type mice. Discussion: The access to exercise wheels provided an initial weight loss in Cpe fat/fat mice, but this effect was offset by elevated food consumption. The serum proteomics results indicated that Cpe fat/fat and wild-type mice differed in their response to exercise.

Original languageEnglish (US)
Pages (from-to)1179-1188
Number of pages10
JournalObesity Research
Volume12
Issue number7
StatePublished - 2004

Fingerprint

Proteomics
proteomics
exercise
Fats
mice
lipids
Serum
wheels
food consumption
Food
Body Weight
blood proteins
body weight
Blood Proteins
carboxypeptidase E
Carboxypeptidase H
kallikreins
Weights and Measures
Kallikreins
Haptoglobins

Keywords

  • Carboxypeptidase E
  • Haptoglobin
  • Kallikrein
  • Neuropeptide
  • Peptide processing

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Effect of voluntary exercise on genetically obese Cpe fat/fat mice : Quantitative proteomics of serum. / Yuan, Quan; Fontenele-Neto, Jose D.; Fricker, Lloyd D.

In: Obesity Research, Vol. 12, No. 7, 2004, p. 1179-1188.

Research output: Contribution to journalArticle

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