Effect of somatic mutation on antibody affinity and specificity

N. Chien, A. M. Giusti, D. Zack, D. French, S. U. Shin, B. Diamond, Matthew D. Scharff

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

By using antibody-forming cells in culture, it is possible to examine the effect of somatic mutations in the variable-region genes of antibodies in the absence of the selective forces that exist in vivo. Such studies reveal that single amino acid substitutions in the variable region of the heavy chain can lead to loss of antigen binding. An example is given of an important protective antibody against a bacterial antigen that undergoes a point mutation, resulting in loss of antigen binding and acquisition of autoreactivity.

Original languageEnglish (US)
Pages (from-to)181-186
Number of pages6
JournalMount Sinai Journal of Medicine
Volume53
Issue number3
StatePublished - 1986
Externally publishedYes

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Antibody Affinity
Antibody Specificity
Mutation
Antibodies
Bacterial Antigens
Antigens
Amino Acid Substitution
Point Mutation
Cell Culture Techniques
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chien, N., Giusti, A. M., Zack, D., French, D., Shin, S. U., Diamond, B., & Scharff, M. D. (1986). Effect of somatic mutation on antibody affinity and specificity. Mount Sinai Journal of Medicine, 53(3), 181-186.

Effect of somatic mutation on antibody affinity and specificity. / Chien, N.; Giusti, A. M.; Zack, D.; French, D.; Shin, S. U.; Diamond, B.; Scharff, Matthew D.

In: Mount Sinai Journal of Medicine, Vol. 53, No. 3, 1986, p. 181-186.

Research output: Contribution to journalArticle

Chien, N, Giusti, AM, Zack, D, French, D, Shin, SU, Diamond, B & Scharff, MD 1986, 'Effect of somatic mutation on antibody affinity and specificity', Mount Sinai Journal of Medicine, vol. 53, no. 3, pp. 181-186.
Chien N, Giusti AM, Zack D, French D, Shin SU, Diamond B et al. Effect of somatic mutation on antibody affinity and specificity. Mount Sinai Journal of Medicine. 1986;53(3):181-186.
Chien, N. ; Giusti, A. M. ; Zack, D. ; French, D. ; Shin, S. U. ; Diamond, B. ; Scharff, Matthew D. / Effect of somatic mutation on antibody affinity and specificity. In: Mount Sinai Journal of Medicine. 1986 ; Vol. 53, No. 3. pp. 181-186.
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