Effect of solvent viscosity on the heme-pocket dynamics of photolyzed (carbonmonoxy)hemoglobin

E. W. Findsen, Joel M. Friedman, M. R. Ondrias

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The heme-pocket dynamics subsequent to carbon monoxide photolysis from human hemoglobin have been monitored as a function of glycerol-water solvent composition with time-resolved resonance Raman spectroscopy. Prompt (geminate) ligand recombination rates and the transient heme-pocket geometry established within 10 ns after photolysis appear to be largely independent of solvent composition. The rate of relaxation of the transient geometry to an equilibrium deoxy configuration is, however, quite sensitive to solvent composition. These observations suggest that the former processes result from local, internal motions of the protein, while the relaxation dynamics of the proximal heme pocket are predicated upon more global protein motions that are dependent upon solvent viscosity.

Original languageEnglish (US)
Pages (from-to)8719-8724
Number of pages6
JournalBiochemistry
Volume27
Issue number24
StatePublished - 1988
Externally publishedYes

Fingerprint

Heme
Viscosity
Hemoglobins
Photolysis
Chemical analysis
Geometry
Raman Spectrum Analysis
Carbon Monoxide
Glycerol
Genetic Recombination
Raman spectroscopy
Proteins
Ligands
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effect of solvent viscosity on the heme-pocket dynamics of photolyzed (carbonmonoxy)hemoglobin. / Findsen, E. W.; Friedman, Joel M.; Ondrias, M. R.

In: Biochemistry, Vol. 27, No. 24, 1988, p. 8719-8724.

Research output: Contribution to journalArticle

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