Effect of phloretin on the permeability of thin lipid membranes

O. Sparre Andersen, A. Finkelstein, I. Katz, A. Cass

Research output: Contribution to journalArticle

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Abstract

Phloretin dramatically increases cation conductances and decreases anion conductances of membranes treated with ion carriers (nonactin, valinomycin, carbonyl cyanide m chlorophenylhydrazone [CCCP], and Hg(C6F5)2) or lipophilic ions (tetraphenylarsonium [TPhAs+] and tetraphenylborate [TPhB-]). For example, on phosphatidylethanolamine membranes, 10-4 M phloretin increases K+ nonactin and TPhAs+ conductances and decreases CCCP- and TPhB- conductances 103 fold; on lecithin:cholesterol membranes, it increases K+ nonactin conductance 105 fold and decreases CCCP- conductance 103 fold. Similar effects are obtained with p and m nitrophenol at 10-2 M. These effects are produced by the un ionized form of phloretin and the nitrophenols. We believe that phloretin, which possesses a large dipole moment, adsorbs and orients at the membrane surface to introduce a dipole potential of opposite polarity to the preexisting positive one, thus increasing the partition coefficient of cations into the membrane interior and decreasing the partition coefficient of anions. (Phloretin may also increase the fluidity of cholesterol containing membranes; this is manifested by its two to three fold increase in nonelectrolyte permeability and its asymmetrical effect on cation and anion conductances in cholesterol containing membranes). It is possible that phloretin's inhibition of chloride, urea, and glucose transport in biological membranes results from the effects of these intense interfacial dipole fields on the translocator(s) of these molecules.

Original languageEnglish (US)
Pages (from-to)749-771
Number of pages23
JournalJournal of General Physiology
Volume67
Issue number6
StatePublished - 1976

Fingerprint

Phloretin
Membrane Lipids
Permeability
Membranes
Tetraphenylborate
Anions
Cations
Cholesterol
Biological Transport
Ions
Valinomycin
Nitrophenols
Lecithins
Urea
Chlorides
Glucose

ASJC Scopus subject areas

  • Physiology

Cite this

Sparre Andersen, O., Finkelstein, A., Katz, I., & Cass, A. (1976). Effect of phloretin on the permeability of thin lipid membranes. Journal of General Physiology, 67(6), 749-771.

Effect of phloretin on the permeability of thin lipid membranes. / Sparre Andersen, O.; Finkelstein, A.; Katz, I.; Cass, A.

In: Journal of General Physiology, Vol. 67, No. 6, 1976, p. 749-771.

Research output: Contribution to journalArticle

Sparre Andersen, O, Finkelstein, A, Katz, I & Cass, A 1976, 'Effect of phloretin on the permeability of thin lipid membranes', Journal of General Physiology, vol. 67, no. 6, pp. 749-771.
Sparre Andersen O, Finkelstein A, Katz I, Cass A. Effect of phloretin on the permeability of thin lipid membranes. Journal of General Physiology. 1976;67(6):749-771.
Sparre Andersen, O. ; Finkelstein, A. ; Katz, I. ; Cass, A. / Effect of phloretin on the permeability of thin lipid membranes. In: Journal of General Physiology. 1976 ; Vol. 67, No. 6. pp. 749-771.
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