Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing

Joo Hee Park, Ja Yeon Kim, Dong Ju Kim, Martha Kim, Minwook Chang, Roy S. Chuck, Choul Yong Park

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Although the wound healing effects of nitric oxide (NO) are known, the mechanism by which NO modulates corneal wound healing remains unclear. In this study, we investigated the effect of exogenous NO donor (NaNO2) on corneal wound healing. We found that NaNO2 (0.1 μM to 100 μM) increased human corneal epithelial cell (HCEC) viability and migration. It also modulated the phosphorylation of mitogen-activated protein kinases (MAPKs) in a time-dependent manner in those HCECs. Further, p38 MAPK phosphorylation increased at 6 h and normalized at 24 h, while the phosphorylation of extracellular signal regulated kinase (ERK) was increased both at 6 h and 24 h. Topical treatment with NaNO2 (10 μM) enhanced corneal epithelial healing and decreased corneal opacity in murine corneal alkali burn model by modulating inflammatory cytokines. Our findings suggest that NO increased HCEC proliferation and migration via time-dependent MAPK activation and eventually enhanced corneal recovery from the alkali burn.

Original languageEnglish (US)
Article number8093
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Wound Healing
Cell Survival
Nitric Oxide
Epithelial Cells
Phosphorylation
Alkalies
Mitogen-Activated Protein Kinases
Cell Movement
Corneal Opacity
Nitric Oxide Donors
Extracellular Signal-Regulated MAP Kinases
p38 Mitogen-Activated Protein Kinases
Cell Proliferation
Cytokines
3-hydroxycholest-7-ene-14-carbaldehyde

ASJC Scopus subject areas

  • General

Cite this

Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing. / Park, Joo Hee; Kim, Ja Yeon; Kim, Dong Ju; Kim, Martha; Chang, Minwook; Chuck, Roy S.; Park, Choul Yong.

In: Scientific Reports, Vol. 7, No. 1, 8093, 01.12.2017.

Research output: Contribution to journalArticle

Park, Joo Hee ; Kim, Ja Yeon ; Kim, Dong Ju ; Kim, Martha ; Chang, Minwook ; Chuck, Roy S. ; Park, Choul Yong. / Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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