Effect of interferon on dimethyl sulfoxide stimulated Friend erythroleukemic cells: ultrastructural and biochemical study

R. B. Luftig, J. F. Conscience, A. Skoultchi, P. McMillan, M. Revel, F. H. Ruddle

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Treatment of dimethyl sulfoxide stimulated Friend erythroleukemic cells (clone 745) with mouse interferon (50 U/ml) led to the following changes: a net decrease (40 to 60%) in both the total number of apparently newly synthesized virion particles per cell section and in the average number of cell sections containing one or more virion particles; a large decrease (80 to 90%) in the number of particles released into the supernatant fluid, as assayed by reverse transcriptase activity; an initial increase in the number of 'immature' or 'enveloped A-type' virions followed by an increase in the accumulation of empty, core shell-like particles; and a decrease in the number of cytoplasmic vacuolar structures, which have been implicated as a major site of virus production and which they show here by serial sectioning to be, in several instances, invaginations of the plasma membrane. The effects on virus production were noticeable 2 h after interferon addition and reached their full extent by 13 h. It is concluded from these observations that interferon acts upon the late stage(s) of virion maturation, leading both to a decrease in virion production as well as to the formation of defective particles. In contrast, a small but significant increase in the rate at which globin mRNA and hemoglobin accumulate is observed after interferon treatment.

Original languageEnglish (US)
Pages (from-to)799-810
Number of pages12
JournalUnknown Journal
Volume23
Issue number3
DOIs
StatePublished - 1977
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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