Abstract
Treatment of dimethyl sulfoxide stimulated Friend erythroleukemic cells (clone 745) with mouse interferon (50 U/ml) led to the following changes: a net decrease (40 to 60%) in both the total number of apparently newly synthesized virion particles per cell section and in the average number of cell sections containing one or more virion particles; a large decrease (80 to 90%) in the number of particles released into the supernatant fluid, as assayed by reverse transcriptase activity; an initial increase in the number of 'immature' or 'enveloped A-type' virions followed by an increase in the accumulation of empty, core shell-like particles; and a decrease in the number of cytoplasmic vacuolar structures, which have been implicated as a major site of virus production and which they show here by serial sectioning to be, in several instances, invaginations of the plasma membrane. The effects on virus production were noticeable 2 h after interferon addition and reached their full extent by 13 h. It is concluded from these observations that interferon acts upon the late stage(s) of virion maturation, leading both to a decrease in virion production as well as to the formation of defective particles. In contrast, a small but significant increase in the rate at which globin mRNA and hemoglobin accumulate is observed after interferon treatment.
Original language | English (US) |
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Pages (from-to) | 799-810 |
Number of pages | 12 |
Journal | Unknown Journal |
Volume | 23 |
Issue number | 3 |
DOIs | |
State | Published - 1977 |
Externally published | Yes |
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology