Effect of haluronan-supplemented dialysate on in vitro function of human peritoneal mesothelial cells

Andrzej Brȩborowicz, Malgorzata Pyda, James Moberly, Leo Martis, Dimitrios Oreopoulos

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background: Addition of hyaluronan (HA) to the dialysis solution has been suggested as a means to protect the peritoneum from injury during peritoneal dialysis (PD). Methods: Concentrations of inflammatory mediators were determined in dialysate samples obtained from PD patients after 6-hour dwells with glucose-based (13.6 g/l) solution containing 0.1 and 0.5 g/l of exogenous high-molecular-weight HA. We additionally evaluated the effect of HA-supplemented dialysate, drained after dwell in PD patients, on function of human peritoneal mesothelial cells (MC) in in vitro culture. Results: Concentration of nitrites was significantly higher in HA 0.5 g/l supplemented dialysate (+43%, p < 0.05) as compared to control. Levels of monocyte chemoattractant protein (MCP-1), soluble intercellular adhesive molecule (s-ICAM), vascular endothelial growth factor (VEGF) and fibronectin were comparable in all the studied groups. However, when MC were exposed in in vitro conditions for 24 h to the studied dialysates, we observed that HA containing fluids inhibited the synthesis of MCP-1, s-ICAM, VEGF and fibronectin in these cells. HA-supplemented dialysate accelerated growth rate of in vitro proliferating MC. Conclusion: High-molecular-weight HA added to the dialysis fluid exerts anti-inflammatory and antifibrotic actions on the in vitro cultured MC and accelerates their growth rate what may be important for peritoneal healing during PD.

Original languageEnglish (US)
Pages (from-to)316-321
Number of pages6
JournalAmerican Journal of Nephrology
Volume24
Issue number3
DOIs
StatePublished - Aug 26 2004

Keywords

  • Hyaluronan
  • Mesothelium
  • Peritoneal dialysis

ASJC Scopus subject areas

  • Nephrology

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