Effect of haluronan-supplemented dialysate on in vitro function of human peritoneal mesothelial cells

Andrzej Brȩborowicz, Malgorzata Pyda, James Moberly, Leo Martis, Dimitrios Oreopoulos

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Addition of hyaluronan (HA) to the dialysis solution has been suggested as a means to protect the peritoneum from injury during peritoneal dialysis (PD). Methods: Concentrations of inflammatory mediators were determined in dialysate samples obtained from PD patients after 6-hour dwells with glucose-based (13.6 g/l) solution containing 0.1 and 0.5 g/l of exogenous high-molecular-weight HA. We additionally evaluated the effect of HA-supplemented dialysate, drained after dwell in PD patients, on function of human peritoneal mesothelial cells (MC) in in vitro culture. Results: Concentration of nitrites was significantly higher in HA 0.5 g/l supplemented dialysate (+43%, p <0.05) as compared to control. Levels of monocyte chemoattractant protein (MCP-1), soluble intercellular adhesive molecule (s-ICAM), vascular endothelial growth factor (VEGF) and fibronectin were comparable in all the studied groups. However, when MC were exposed in in vitro conditions for 24 h to the studied dialysates, we observed that HA containing fluids inhibited the synthesis of MCP-1, s-ICAM, VEGF and fibronectin in these cells. HA-supplemented dialysate accelerated growth rate of in vitro proliferating MC. Conclusion: High-molecular-weight HA added to the dialysis fluid exerts anti-inflammatory and antifibrotic actions on the in vitro cultured MC and accelerates their growth rate what may be important for peritoneal healing during PD.

Original languageEnglish (US)
Pages (from-to)316-321
Number of pages6
JournalAmerican Journal of Nephrology
Volume24
Issue number3
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Dialysis Solutions
Hyaluronic Acid
Peritoneal Dialysis
Fibronectins
Adhesives
Vascular Endothelial Growth Factor A
Molecular Weight
Chemokine CCL2
Peritoneum
Growth
Nitrites
In Vitro Techniques
Dialysis
Cultured Cells
Anti-Inflammatory Agents
Glucose
Wounds and Injuries

Keywords

  • Hyaluronan
  • Mesothelium
  • Peritoneal dialysis

ASJC Scopus subject areas

  • Nephrology

Cite this

Effect of haluronan-supplemented dialysate on in vitro function of human peritoneal mesothelial cells. / Brȩborowicz, Andrzej; Pyda, Malgorzata; Moberly, James; Martis, Leo; Oreopoulos, Dimitrios.

In: American Journal of Nephrology, Vol. 24, No. 3, 2004, p. 316-321.

Research output: Contribution to journalArticle

Brȩborowicz, Andrzej ; Pyda, Malgorzata ; Moberly, James ; Martis, Leo ; Oreopoulos, Dimitrios. / Effect of haluronan-supplemented dialysate on in vitro function of human peritoneal mesothelial cells. In: American Journal of Nephrology. 2004 ; Vol. 24, No. 3. pp. 316-321.
@article{d39e27c837ec4df18d3f108ece57eed7,
title = "Effect of haluronan-supplemented dialysate on in vitro function of human peritoneal mesothelial cells",
abstract = "Background: Addition of hyaluronan (HA) to the dialysis solution has been suggested as a means to protect the peritoneum from injury during peritoneal dialysis (PD). Methods: Concentrations of inflammatory mediators were determined in dialysate samples obtained from PD patients after 6-hour dwells with glucose-based (13.6 g/l) solution containing 0.1 and 0.5 g/l of exogenous high-molecular-weight HA. We additionally evaluated the effect of HA-supplemented dialysate, drained after dwell in PD patients, on function of human peritoneal mesothelial cells (MC) in in vitro culture. Results: Concentration of nitrites was significantly higher in HA 0.5 g/l supplemented dialysate (+43{\%}, p <0.05) as compared to control. Levels of monocyte chemoattractant protein (MCP-1), soluble intercellular adhesive molecule (s-ICAM), vascular endothelial growth factor (VEGF) and fibronectin were comparable in all the studied groups. However, when MC were exposed in in vitro conditions for 24 h to the studied dialysates, we observed that HA containing fluids inhibited the synthesis of MCP-1, s-ICAM, VEGF and fibronectin in these cells. HA-supplemented dialysate accelerated growth rate of in vitro proliferating MC. Conclusion: High-molecular-weight HA added to the dialysis fluid exerts anti-inflammatory and antifibrotic actions on the in vitro cultured MC and accelerates their growth rate what may be important for peritoneal healing during PD.",
keywords = "Hyaluronan, Mesothelium, Peritoneal dialysis",
author = "Andrzej Brȩborowicz and Malgorzata Pyda and James Moberly and Leo Martis and Dimitrios Oreopoulos",
year = "2004",
doi = "10.1159/000078463",
language = "English (US)",
volume = "24",
pages = "316--321",
journal = "American Journal of Nephrology",
issn = "0250-8095",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Effect of haluronan-supplemented dialysate on in vitro function of human peritoneal mesothelial cells

AU - Brȩborowicz, Andrzej

AU - Pyda, Malgorzata

AU - Moberly, James

AU - Martis, Leo

AU - Oreopoulos, Dimitrios

PY - 2004

Y1 - 2004

N2 - Background: Addition of hyaluronan (HA) to the dialysis solution has been suggested as a means to protect the peritoneum from injury during peritoneal dialysis (PD). Methods: Concentrations of inflammatory mediators were determined in dialysate samples obtained from PD patients after 6-hour dwells with glucose-based (13.6 g/l) solution containing 0.1 and 0.5 g/l of exogenous high-molecular-weight HA. We additionally evaluated the effect of HA-supplemented dialysate, drained after dwell in PD patients, on function of human peritoneal mesothelial cells (MC) in in vitro culture. Results: Concentration of nitrites was significantly higher in HA 0.5 g/l supplemented dialysate (+43%, p <0.05) as compared to control. Levels of monocyte chemoattractant protein (MCP-1), soluble intercellular adhesive molecule (s-ICAM), vascular endothelial growth factor (VEGF) and fibronectin were comparable in all the studied groups. However, when MC were exposed in in vitro conditions for 24 h to the studied dialysates, we observed that HA containing fluids inhibited the synthesis of MCP-1, s-ICAM, VEGF and fibronectin in these cells. HA-supplemented dialysate accelerated growth rate of in vitro proliferating MC. Conclusion: High-molecular-weight HA added to the dialysis fluid exerts anti-inflammatory and antifibrotic actions on the in vitro cultured MC and accelerates their growth rate what may be important for peritoneal healing during PD.

AB - Background: Addition of hyaluronan (HA) to the dialysis solution has been suggested as a means to protect the peritoneum from injury during peritoneal dialysis (PD). Methods: Concentrations of inflammatory mediators were determined in dialysate samples obtained from PD patients after 6-hour dwells with glucose-based (13.6 g/l) solution containing 0.1 and 0.5 g/l of exogenous high-molecular-weight HA. We additionally evaluated the effect of HA-supplemented dialysate, drained after dwell in PD patients, on function of human peritoneal mesothelial cells (MC) in in vitro culture. Results: Concentration of nitrites was significantly higher in HA 0.5 g/l supplemented dialysate (+43%, p <0.05) as compared to control. Levels of monocyte chemoattractant protein (MCP-1), soluble intercellular adhesive molecule (s-ICAM), vascular endothelial growth factor (VEGF) and fibronectin were comparable in all the studied groups. However, when MC were exposed in in vitro conditions for 24 h to the studied dialysates, we observed that HA containing fluids inhibited the synthesis of MCP-1, s-ICAM, VEGF and fibronectin in these cells. HA-supplemented dialysate accelerated growth rate of in vitro proliferating MC. Conclusion: High-molecular-weight HA added to the dialysis fluid exerts anti-inflammatory and antifibrotic actions on the in vitro cultured MC and accelerates their growth rate what may be important for peritoneal healing during PD.

KW - Hyaluronan

KW - Mesothelium

KW - Peritoneal dialysis

UR - http://www.scopus.com/inward/record.url?scp=4043171989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4043171989&partnerID=8YFLogxK

U2 - 10.1159/000078463

DO - 10.1159/000078463

M3 - Article

VL - 24

SP - 316

EP - 321

JO - American Journal of Nephrology

JF - American Journal of Nephrology

SN - 0250-8095

IS - 3

ER -