Effect of ganaxolone on flurothyl seizures in developing rats

Soňa Liptáková, Libor Velíšek, Jana Velíšková, Solomon L. Moshe

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Purpose: To determine the effects of a newly synthesized epalon, ganaxolone (GNX), on primarily generalized seizures in rats of various ages during development. Epalons are classified as neuroactive steroids that interact at unique site of the GABA(A) receptor-Cl(-) channel complex in the central nervous system. Methods: Sprague-Dawley male rats were used at 9, 15, 30, and 60 postnatal days (PN), GNX dissolved in 2-hydroxypropyl-β- cyclodextrine was administered intraperitoneally in different doses at various time points before flurothyl testing. The incidence and threshold of clonic and tonic-clonic flurothyl seizures were evaluated. Behavioral changes were also assessed. Results: In all age groups, the effects of GNX were dose dependent and more prominent 10 min after its administration. In PN 60 and PN 30 rats, GNX had dose-dependent anticonvulsant effects; tonic-clonic seizures were more sensitive to GNX treatment than clonic seizures. In PN 15 and PN 9 rats, GNX demonstrated dose- and time-dependent anticonvulsant effects against both types of flurothyl-induced seizures. GNX was more effective in PN 15 rats than in other age groups, but at doses that altered motor behavior. Conclusions: GNX has anticonvulsant effects against flurothyl- induced seizures in all age groups tested. Its effects are more prominent in the two younger age groups, especially in PN 15 rats, but are associated with motor side effects.

Original languageEnglish (US)
Pages (from-to)788-793
Number of pages6
JournalEpilepsia
Volume41
Issue number7
StatePublished - 2000

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Flurothyl
Seizures
Age Groups
Anticonvulsants
ganaxolone
GABA-A Receptors
Sprague Dawley Rats
Central Nervous System
Steroids

Keywords

  • Development
  • Flurothyl
  • Ganaxolone
  • Rats
  • Seizures

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Liptáková, S., Velíšek, L., Velíšková, J., & Moshe, S. L. (2000). Effect of ganaxolone on flurothyl seizures in developing rats. Epilepsia, 41(7), 788-793.

Effect of ganaxolone on flurothyl seizures in developing rats. / Liptáková, Soňa; Velíšek, Libor; Velíšková, Jana; Moshe, Solomon L.

In: Epilepsia, Vol. 41, No. 7, 2000, p. 788-793.

Research output: Contribution to journalArticle

Liptáková, S, Velíšek, L, Velíšková, J & Moshe, SL 2000, 'Effect of ganaxolone on flurothyl seizures in developing rats', Epilepsia, vol. 41, no. 7, pp. 788-793.
Liptáková S, Velíšek L, Velíšková J, Moshe SL. Effect of ganaxolone on flurothyl seizures in developing rats. Epilepsia. 2000;41(7):788-793.
Liptáková, Soňa ; Velíšek, Libor ; Velíšková, Jana ; Moshe, Solomon L. / Effect of ganaxolone on flurothyl seizures in developing rats. In: Epilepsia. 2000 ; Vol. 41, No. 7. pp. 788-793.
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