Effect of copy number variants on outcomes for infants with single ventricle heart defects

Abigail S. Carey, Li Liang, Jonathan Edwards, Tracy Brandt, Hui Mei, Andrew J. Sharp, Daphne T. Hsu, Jane W. Newburger, Richard G. Ohye, Wendy K. Chung, Mark W. Russell, Jill A. Rosenfeld, Lisa G. Shaffer, Michael K. Parides, Lisa Edelmann, Bruce D. Gelb

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background-Human genomes harbor copy number variants (CNVs), which are regions of DNA gains or losses. Although pathogenic CNVs are associated with congenital heart disease (CHD), their effect on clinical outcomes is unknown. This study sought to determine whether pathogenic CNVs among infants with single ventricle physiology were associated with inferior neurocognitive and somatic growth outcomes. Methods and Results-Genomic DNAs from 223 subjects of 2 National Heart, Lung, and Blood Institute-sponsored randomized clinical trials in infants with single ventricle CHD and 270 controls from The Cancer Genome Atlas project were analyzed for rare CNVs <300 kb using array comparative genomic hybridization. Neurocognitive and growth outcomes at 14 months from the CHD trials were compared among subjects with and without pathogenic CNVs. Putatively pathogenic CNVs, comprising 25 duplications and 6 deletions, had a prevalence of 13.9%, significantly greater than the 4.4% rate of such CNVs among controls. CNVs associated with genomic disorders were found in 13 cases but not in controls. Several CNVs likely to be causative of single ventricle CHD were observed, including aberrations altering the dosage of GATA4, MYH11, and GJA5. Subjects with pathogenic CNVs had worse linear growth, and those with CNVs associated with known genomic disorders had the poorest neurocognitive and growth outcomes. A minority of children with pathogenic CNVs were noted to be dysmorphic on clinical genetics examination. Conclusions-Pathogenic CNVs seem to contribute to the cause of single ventricle forms of CHD in =10% of cases and are clinically subtle but adversely affect outcomes in children harboring them.

Original languageEnglish (US)
Pages (from-to)444-451
Number of pages8
JournalCirculation: Cardiovascular Genetics
Volume6
Issue number5
DOIs
StatePublished - Oct 2013
Externally publishedYes

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Heart Ventricles
Heart Diseases
Growth
National Heart, Lung, and Blood Institute (U.S.)
Comparative Genomic Hybridization
Atlases
DNA
Human Genome
Randomized Controlled Trials
Genome
Neoplasms

Keywords

  • Congenital cardiac defect
  • DNA copy number variantiations
  • Hypoplastic left heart syndrome
  • Outcome

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Genetics(clinical)
  • Genetics

Cite this

Carey, A. S., Liang, L., Edwards, J., Brandt, T., Mei, H., Sharp, A. J., ... Gelb, B. D. (2013). Effect of copy number variants on outcomes for infants with single ventricle heart defects. Circulation: Cardiovascular Genetics, 6(5), 444-451. https://doi.org/10.1161/CIRCGENETICS.113.000189

Effect of copy number variants on outcomes for infants with single ventricle heart defects. / Carey, Abigail S.; Liang, Li; Edwards, Jonathan; Brandt, Tracy; Mei, Hui; Sharp, Andrew J.; Hsu, Daphne T.; Newburger, Jane W.; Ohye, Richard G.; Chung, Wendy K.; Russell, Mark W.; Rosenfeld, Jill A.; Shaffer, Lisa G.; Parides, Michael K.; Edelmann, Lisa; Gelb, Bruce D.

In: Circulation: Cardiovascular Genetics, Vol. 6, No. 5, 10.2013, p. 444-451.

Research output: Contribution to journalArticle

Carey, AS, Liang, L, Edwards, J, Brandt, T, Mei, H, Sharp, AJ, Hsu, DT, Newburger, JW, Ohye, RG, Chung, WK, Russell, MW, Rosenfeld, JA, Shaffer, LG, Parides, MK, Edelmann, L & Gelb, BD 2013, 'Effect of copy number variants on outcomes for infants with single ventricle heart defects', Circulation: Cardiovascular Genetics, vol. 6, no. 5, pp. 444-451. https://doi.org/10.1161/CIRCGENETICS.113.000189
Carey, Abigail S. ; Liang, Li ; Edwards, Jonathan ; Brandt, Tracy ; Mei, Hui ; Sharp, Andrew J. ; Hsu, Daphne T. ; Newburger, Jane W. ; Ohye, Richard G. ; Chung, Wendy K. ; Russell, Mark W. ; Rosenfeld, Jill A. ; Shaffer, Lisa G. ; Parides, Michael K. ; Edelmann, Lisa ; Gelb, Bruce D. / Effect of copy number variants on outcomes for infants with single ventricle heart defects. In: Circulation: Cardiovascular Genetics. 2013 ; Vol. 6, No. 5. pp. 444-451.
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abstract = "Background-Human genomes harbor copy number variants (CNVs), which are regions of DNA gains or losses. Although pathogenic CNVs are associated with congenital heart disease (CHD), their effect on clinical outcomes is unknown. This study sought to determine whether pathogenic CNVs among infants with single ventricle physiology were associated with inferior neurocognitive and somatic growth outcomes. Methods and Results-Genomic DNAs from 223 subjects of 2 National Heart, Lung, and Blood Institute-sponsored randomized clinical trials in infants with single ventricle CHD and 270 controls from The Cancer Genome Atlas project were analyzed for rare CNVs <300 kb using array comparative genomic hybridization. Neurocognitive and growth outcomes at 14 months from the CHD trials were compared among subjects with and without pathogenic CNVs. Putatively pathogenic CNVs, comprising 25 duplications and 6 deletions, had a prevalence of 13.9{\%}, significantly greater than the 4.4{\%} rate of such CNVs among controls. CNVs associated with genomic disorders were found in 13 cases but not in controls. Several CNVs likely to be causative of single ventricle CHD were observed, including aberrations altering the dosage of GATA4, MYH11, and GJA5. Subjects with pathogenic CNVs had worse linear growth, and those with CNVs associated with known genomic disorders had the poorest neurocognitive and growth outcomes. A minority of children with pathogenic CNVs were noted to be dysmorphic on clinical genetics examination. Conclusions-Pathogenic CNVs seem to contribute to the cause of single ventricle forms of CHD in =10{\%} of cases and are clinically subtle but adversely affect outcomes in children harboring them.",
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AU - Carey, Abigail S.

AU - Liang, Li

AU - Edwards, Jonathan

AU - Brandt, Tracy

AU - Mei, Hui

AU - Sharp, Andrew J.

AU - Hsu, Daphne T.

AU - Newburger, Jane W.

AU - Ohye, Richard G.

AU - Chung, Wendy K.

AU - Russell, Mark W.

AU - Rosenfeld, Jill A.

AU - Shaffer, Lisa G.

AU - Parides, Michael K.

AU - Edelmann, Lisa

AU - Gelb, Bruce D.

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N2 - Background-Human genomes harbor copy number variants (CNVs), which are regions of DNA gains or losses. Although pathogenic CNVs are associated with congenital heart disease (CHD), their effect on clinical outcomes is unknown. This study sought to determine whether pathogenic CNVs among infants with single ventricle physiology were associated with inferior neurocognitive and somatic growth outcomes. Methods and Results-Genomic DNAs from 223 subjects of 2 National Heart, Lung, and Blood Institute-sponsored randomized clinical trials in infants with single ventricle CHD and 270 controls from The Cancer Genome Atlas project were analyzed for rare CNVs <300 kb using array comparative genomic hybridization. Neurocognitive and growth outcomes at 14 months from the CHD trials were compared among subjects with and without pathogenic CNVs. Putatively pathogenic CNVs, comprising 25 duplications and 6 deletions, had a prevalence of 13.9%, significantly greater than the 4.4% rate of such CNVs among controls. CNVs associated with genomic disorders were found in 13 cases but not in controls. Several CNVs likely to be causative of single ventricle CHD were observed, including aberrations altering the dosage of GATA4, MYH11, and GJA5. Subjects with pathogenic CNVs had worse linear growth, and those with CNVs associated with known genomic disorders had the poorest neurocognitive and growth outcomes. A minority of children with pathogenic CNVs were noted to be dysmorphic on clinical genetics examination. Conclusions-Pathogenic CNVs seem to contribute to the cause of single ventricle forms of CHD in =10% of cases and are clinically subtle but adversely affect outcomes in children harboring them.

AB - Background-Human genomes harbor copy number variants (CNVs), which are regions of DNA gains or losses. Although pathogenic CNVs are associated with congenital heart disease (CHD), their effect on clinical outcomes is unknown. This study sought to determine whether pathogenic CNVs among infants with single ventricle physiology were associated with inferior neurocognitive and somatic growth outcomes. Methods and Results-Genomic DNAs from 223 subjects of 2 National Heart, Lung, and Blood Institute-sponsored randomized clinical trials in infants with single ventricle CHD and 270 controls from The Cancer Genome Atlas project were analyzed for rare CNVs <300 kb using array comparative genomic hybridization. Neurocognitive and growth outcomes at 14 months from the CHD trials were compared among subjects with and without pathogenic CNVs. Putatively pathogenic CNVs, comprising 25 duplications and 6 deletions, had a prevalence of 13.9%, significantly greater than the 4.4% rate of such CNVs among controls. CNVs associated with genomic disorders were found in 13 cases but not in controls. Several CNVs likely to be causative of single ventricle CHD were observed, including aberrations altering the dosage of GATA4, MYH11, and GJA5. Subjects with pathogenic CNVs had worse linear growth, and those with CNVs associated with known genomic disorders had the poorest neurocognitive and growth outcomes. A minority of children with pathogenic CNVs were noted to be dysmorphic on clinical genetics examination. Conclusions-Pathogenic CNVs seem to contribute to the cause of single ventricle forms of CHD in =10% of cases and are clinically subtle but adversely affect outcomes in children harboring them.

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KW - DNA copy number variantiations

KW - Hypoplastic left heart syndrome

KW - Outcome

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