@article{ee6ffb5cc71e4e3a94bf27e445704da7,
title = "Effect of cell seeding on neotissue formation in a tissue engineered trachea",
abstract = "Background Surgical management of long segment tracheal disease is limited by a paucity of donor tissue and poor performance of synthetic materials. A potential solution is the development of a tissue-engineered tracheal graft (TETG) which promises an autologous airway conduit with growth capacity. Methods We created a TETG by vacuum seeding bone marrow-derived mononuclear cells (BM-MNCs) on a polymeric nanofiber scaffold. First, we evaluated the role of scaffold porosity on cell seeding efficiency in vitro. We then determined the effect of cell seeding on graft performance in vivo using an ovine model. Results Seeding efficiency of normal porosity (NP) grafts was significantly increased when compared to high porosity (HP) grafts (NP: 360.3 ± 69.19 × 103 cells/mm2; HP: 133.7 ± 22.73 × 103 cells/mm2; p < 0.004). Lambs received unseeded (n = 2) or seeded (n = 3) NP scaffolds as tracheal interposition grafts for 6 weeks. Three animals were terminated early owing to respiratory complications (n = 2 unseeded, n = 1 seeded). Seeded TETG explants demonstrated wound healing, epithelial migration, and delayed stenosis when compared to their unseeded counterparts. Conclusion Vacuum seeding BM-MNCs on nanofiber scaffolds for immediate implantation as tracheal interposition grafts is a viable approach to generate TETGs, but further preclinical research is warranted before advocating this technology for clinical application.",
keywords = "Bone marrow mononuclear cells, Electrospinning, Large animal model, Tissue engineering, Trachea, Vacuum seeding",
author = "Clark, {Elizabeth S.} and Cameron Best and Ekene Onwuka and Tadahisa Sugiura and Nathan Mahler and Brad Bolon and Andrew Niehaus and Iyore James and Narutoshi Hibino and Toshiharu Shinoka and Jed Johnson and Breuer, {Christopher K.}",
note = "Funding Information: The work presented was supported by the National Science Foundation Award # IIP-1315524 (Nanofiber Solutions, Inc.) and T32 OD010429-13 (ESC). The Comparative Pathology and Mouse Phenotyping Shared Resource, Department of Veterinary Biosciences and the Comprehensive Cancer Center are supported in part by grant P30 CA016058. The Morphology Core at Nationwide Children's Hospital Tissue performed tissue processing and H&E staining. Specifically, we would like to thank Dr. Nina Zitzer, the NCH Animal Care staff, Yongjie Miao (Biostatistician for the NCH Cardiovascular and Pulmonary Research Institute), Matt Wiet, Ben Brownback and Josh Meeter (B2 Group) for their assistance. Funding Information: The work presented was supported by the National Science Foundation Award # IIP-1315524 (Nanofiber Solutions, Inc.) and T32 OD010429-13 (ESC). The Comparative Pathology and Mouse Phenotyping Shared Resource, Department of Veterinary Biosciences and the Comprehensive Cancer Center are supported in part by grant P30 CA016058. The Morphology Core at Nationwide Children''s Hospital Tissue performed tissue processing and H&E staining. Specifically, we would like to thank Dr. Nina Zitzer, the NCH Animal Care staff, Yongjie Miao (Biostatistician for the NCH Cardiovascular and Pulmonary Research Institute), Matt Wiet, Ben Brownback and Josh Meeter (B2 Group) for their assistance. Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
month = jan,
day = "1",
doi = "10.1016/j.jpedsurg.2015.10.008",
language = "English (US)",
volume = "51",
pages = "49--55",
journal = "Journal of Pediatric Surgery",
issn = "0022-3468",
publisher = "W.B. Saunders Ltd",
number = "1",
}