Effect of an induced conformational change on the physical properties of two chemotactic receptor molecules

R. Suzanne Zukin, P. R. Hartig, D. E. Koshland

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The physical properties and conformational dynamics of the Salmonella typhimurium ribose and galactose receptors have been examined. Studies involving circular dichroism, fluorescence, absorption spectroscopy, and sedimentation analysis show that the two receptor proteins have different morphologies and exhibit diverse responses to sugar binding. The ribose receptor lacks both tryptophan and disulfide residues, and the galactose receptor lacks disulfides and has only a single tryptophan residue. By virtue of these fortuitous properties, the conformational changes induced in these proteins by sugar binding can be dissected by utilizing a variety of physical probes. A ligand-induced conformational change in the ribose receptor is shown by circular dichroism and fluorescence spectroscopy, which reveal spectral changes assignable to tyrosine, phenylalanine, and methionine residues. A conformational change in the galactose receptor has been demonstrated by fluorescence spectroscopy involving the distant reporter group method, which shows changes assignable to tryptophan and methionine sites and which is corroborated by sedimentation analysis. It is clear that there are extensive conformational changes in the two receptor proteins and that the different physical methods provide complementary information on the nature of these changes.

Original languageEnglish (US)
Pages (from-to)5599-5605
Number of pages7
JournalBiochemistry
Volume18
Issue number25
StatePublished - 1979

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Ribose
Fluorescence Spectrometry
Tryptophan
Physical properties
Fluorescence spectroscopy
Circular Dichroism
Sedimentation
Sugars
Disulfides
Methionine
Molecules
Circular dichroism spectroscopy
Proteins
Salmonella
Salmonella typhimurium
Phenylalanine
Absorption spectroscopy
Protein Binding
Tyrosine
Fluorescence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effect of an induced conformational change on the physical properties of two chemotactic receptor molecules. / Zukin, R. Suzanne; Hartig, P. R.; Koshland, D. E.

In: Biochemistry, Vol. 18, No. 25, 1979, p. 5599-5605.

Research output: Contribution to journalArticle

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