Effect of adenovirus on host cell DNA synthesis in synchronized cells

L. D. Hodge; M. D. Scharff

doi:10.1016/0042-6822(69)90273-6

Effect of adenovirus on host cell DNA synthesis in synchronized cells

L. D. Hodge, M. D. Scharff

Cell Biology

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Viral and cellular DNA synthesis were examined in synchronized HeLa cells infected at various stages in the cell cycle with adenovirus type 2. Viral DNA synthesis began approximately 9 hours after infection, whether cells were infected during S or G₁. When viral DNA was synthesized during G₁, premature replication of cellular DNA did not occur, and its subsequent initiation at the beginning of S was prevented. When viral DNA synthesis began after the onset of S, cellular DNA replication was partially, but not completely, inhibited. Nucleohistones were synthesized only in association with cellular DNA.

Original language	English (US)
Pages (from-to)	554-564
Number of pages	11
Journal	Virology
Volume	37
Issue number	4
DOIs	https://doi.org/10.1016/0042-6822(69)90273-6
State	Published - Apr 1969

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(69)90273-6

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title = "Effect of adenovirus on host cell DNA synthesis in synchronized cells",

abstract = "Viral and cellular DNA synthesis were examined in synchronized HeLa cells infected at various stages in the cell cycle with adenovirus type 2. Viral DNA synthesis began approximately 9 hours after infection, whether cells were infected during S or G1. When viral DNA was synthesized during G1, premature replication of cellular DNA did not occur, and its subsequent initiation at the beginning of S was prevented. When viral DNA synthesis began after the onset of S, cellular DNA replication was partially, but not completely, inhibited. Nucleohistones were synthesized only in association with cellular DNA.",

author = "Hodge, {L. D.} and Scharff, {M. D.}",

note = "Funding Information: ACKNOWLEDGMENTS These investigationsw eres upportedb y grants from the National Science Foundation, the U.S.P.H.S. (AI-4153, AI-5231), and the ACS. Dr. Hodge was supported by a Training Grant from the U.S.P.H.S., and Dr. Scharff is a recipient of U.S.P.H.S. Career Development Award. We would like to thank Dr. Thaddeus Borun for his collaboration in the histone experiment.",

year = "1969",

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doi = "10.1016/0042-6822(69)90273-6",

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T1 - Effect of adenovirus on host cell DNA synthesis in synchronized cells

AU - Hodge, L. D.

AU - Scharff, M. D.

N1 - Funding Information: ACKNOWLEDGMENTS These investigationsw eres upportedb y grants from the National Science Foundation, the U.S.P.H.S. (AI-4153, AI-5231), and the ACS. Dr. Hodge was supported by a Training Grant from the U.S.P.H.S., and Dr. Scharff is a recipient of U.S.P.H.S. Career Development Award. We would like to thank Dr. Thaddeus Borun for his collaboration in the histone experiment.

PY - 1969/4

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N2 - Viral and cellular DNA synthesis were examined in synchronized HeLa cells infected at various stages in the cell cycle with adenovirus type 2. Viral DNA synthesis began approximately 9 hours after infection, whether cells were infected during S or G1. When viral DNA was synthesized during G1, premature replication of cellular DNA did not occur, and its subsequent initiation at the beginning of S was prevented. When viral DNA synthesis began after the onset of S, cellular DNA replication was partially, but not completely, inhibited. Nucleohistones were synthesized only in association with cellular DNA.

AB - Viral and cellular DNA synthesis were examined in synchronized HeLa cells infected at various stages in the cell cycle with adenovirus type 2. Viral DNA synthesis began approximately 9 hours after infection, whether cells were infected during S or G1. When viral DNA was synthesized during G1, premature replication of cellular DNA did not occur, and its subsequent initiation at the beginning of S was prevented. When viral DNA synthesis began after the onset of S, cellular DNA replication was partially, but not completely, inhibited. Nucleohistones were synthesized only in association with cellular DNA.

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Effect of adenovirus on host cell DNA synthesis in synchronized cells

Abstract

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