Effect of β-muricholic acid on the prevention and dissolution of cholesterol gallstones in C57L/J mice

David Q.H. Wang, Susumu Tazuma

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

This study investigated whether β-muricholic acid, a natural trihydroxy hydrophilic bile acid of rodents, acts as a biliary cholesterol-desaturating agent to prevent cholesterol gallstones and if it facilitates the dissolution of gallstones compared with ursodeoxycholic acid (UDCA). For gallstone prevention study, gallstone-susceptible male C57L mice were fed 8 weeks with a lithogenic diet (2% cholesterol and 0.5% cholic acid) with or without 0.5% UDCA or β-muricholic acid. For gallstone dissolution study, additional groups of mice that have formed gallstones were fed chow with or without 0.5% β-muricholic acid or UDCA for 8 weeks. One hundred percent of mice fed the lithogenic diet formed cholesterol gallstones. Addition of β-muricholic acid and UDCA decreased gallstone prevalence to 20% and 50% through significantly reducing biliary secretion rate, saturation index, and intestinal absorption of cholesterol, as well as inducing phase boundary shift and an enlarged Region E that prevented the transition of cholesterol from its liquid crystalline phase to solid crystals and stones. Eight weeks of β-muricholic acid and UDCA administration produced complete gallstone dissolution rates of 100% and 60% compared with the chow (10%). We conclude that β-muricholic acid is more effective than UDCA in treating or preventing diet-induced or experimental cholesterol gallstones in mice.

Original languageEnglish (US)
Pages (from-to)1960-1968
Number of pages9
JournalJournal of Lipid Research
Volume43
Issue number11
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Ursodeoxycholic Acid
Gallstones
Dissolution
Cholesterol
Nutrition
Diet
Cholic Acid
Phase boundaries
muricholic acid
Bile Acids and Salts
Intestinal Absorption
Crystalline materials
Crystals
Rodentia
Liquids

Keywords

  • Bile flow
  • Intestinal cholesterol absorption
  • Phase diagram
  • Phospholipid

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Effect of β-muricholic acid on the prevention and dissolution of cholesterol gallstones in C57L/J mice. / Wang, David Q.H.; Tazuma, Susumu.

In: Journal of Lipid Research, Vol. 43, No. 11, 01.11.2002, p. 1960-1968.

Research output: Contribution to journalArticle

@article{3a7f0db977d14160b36d0332867a75a0,
title = "Effect of β-muricholic acid on the prevention and dissolution of cholesterol gallstones in C57L/J mice",
abstract = "This study investigated whether β-muricholic acid, a natural trihydroxy hydrophilic bile acid of rodents, acts as a biliary cholesterol-desaturating agent to prevent cholesterol gallstones and if it facilitates the dissolution of gallstones compared with ursodeoxycholic acid (UDCA). For gallstone prevention study, gallstone-susceptible male C57L mice were fed 8 weeks with a lithogenic diet (2{\%} cholesterol and 0.5{\%} cholic acid) with or without 0.5{\%} UDCA or β-muricholic acid. For gallstone dissolution study, additional groups of mice that have formed gallstones were fed chow with or without 0.5{\%} β-muricholic acid or UDCA for 8 weeks. One hundred percent of mice fed the lithogenic diet formed cholesterol gallstones. Addition of β-muricholic acid and UDCA decreased gallstone prevalence to 20{\%} and 50{\%} through significantly reducing biliary secretion rate, saturation index, and intestinal absorption of cholesterol, as well as inducing phase boundary shift and an enlarged Region E that prevented the transition of cholesterol from its liquid crystalline phase to solid crystals and stones. Eight weeks of β-muricholic acid and UDCA administration produced complete gallstone dissolution rates of 100{\%} and 60{\%} compared with the chow (10{\%}). We conclude that β-muricholic acid is more effective than UDCA in treating or preventing diet-induced or experimental cholesterol gallstones in mice.",
keywords = "Bile flow, Intestinal cholesterol absorption, Phase diagram, Phospholipid",
author = "Wang, {David Q.H.} and Susumu Tazuma",
year = "2002",
month = "11",
day = "1",
doi = "10.1194/jlr.M200297-JLR200",
language = "English (US)",
volume = "43",
pages = "1960--1968",
journal = "Journal of Lipid Research",
issn = "0022-2275",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "11",

}

TY - JOUR

T1 - Effect of β-muricholic acid on the prevention and dissolution of cholesterol gallstones in C57L/J mice

AU - Wang, David Q.H.

AU - Tazuma, Susumu

PY - 2002/11/1

Y1 - 2002/11/1

N2 - This study investigated whether β-muricholic acid, a natural trihydroxy hydrophilic bile acid of rodents, acts as a biliary cholesterol-desaturating agent to prevent cholesterol gallstones and if it facilitates the dissolution of gallstones compared with ursodeoxycholic acid (UDCA). For gallstone prevention study, gallstone-susceptible male C57L mice were fed 8 weeks with a lithogenic diet (2% cholesterol and 0.5% cholic acid) with or without 0.5% UDCA or β-muricholic acid. For gallstone dissolution study, additional groups of mice that have formed gallstones were fed chow with or without 0.5% β-muricholic acid or UDCA for 8 weeks. One hundred percent of mice fed the lithogenic diet formed cholesterol gallstones. Addition of β-muricholic acid and UDCA decreased gallstone prevalence to 20% and 50% through significantly reducing biliary secretion rate, saturation index, and intestinal absorption of cholesterol, as well as inducing phase boundary shift and an enlarged Region E that prevented the transition of cholesterol from its liquid crystalline phase to solid crystals and stones. Eight weeks of β-muricholic acid and UDCA administration produced complete gallstone dissolution rates of 100% and 60% compared with the chow (10%). We conclude that β-muricholic acid is more effective than UDCA in treating or preventing diet-induced or experimental cholesterol gallstones in mice.

AB - This study investigated whether β-muricholic acid, a natural trihydroxy hydrophilic bile acid of rodents, acts as a biliary cholesterol-desaturating agent to prevent cholesterol gallstones and if it facilitates the dissolution of gallstones compared with ursodeoxycholic acid (UDCA). For gallstone prevention study, gallstone-susceptible male C57L mice were fed 8 weeks with a lithogenic diet (2% cholesterol and 0.5% cholic acid) with or without 0.5% UDCA or β-muricholic acid. For gallstone dissolution study, additional groups of mice that have formed gallstones were fed chow with or without 0.5% β-muricholic acid or UDCA for 8 weeks. One hundred percent of mice fed the lithogenic diet formed cholesterol gallstones. Addition of β-muricholic acid and UDCA decreased gallstone prevalence to 20% and 50% through significantly reducing biliary secretion rate, saturation index, and intestinal absorption of cholesterol, as well as inducing phase boundary shift and an enlarged Region E that prevented the transition of cholesterol from its liquid crystalline phase to solid crystals and stones. Eight weeks of β-muricholic acid and UDCA administration produced complete gallstone dissolution rates of 100% and 60% compared with the chow (10%). We conclude that β-muricholic acid is more effective than UDCA in treating or preventing diet-induced or experimental cholesterol gallstones in mice.

KW - Bile flow

KW - Intestinal cholesterol absorption

KW - Phase diagram

KW - Phospholipid

UR - http://www.scopus.com/inward/record.url?scp=0036845139&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036845139&partnerID=8YFLogxK

U2 - 10.1194/jlr.M200297-JLR200

DO - 10.1194/jlr.M200297-JLR200

M3 - Article

VL - 43

SP - 1960

EP - 1968

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 11

ER -