Early loss of proliferative potential of human peritoneal mesothelial cells in culture

The role of p16INK4a-mediated premature senescence

Krzysztof Ksia̧zek, Katarzyna Piwocka, Agnieszka Brzezińska, Ewa Sikora, Maciej Zabel, Andrzej Brȩborowicz, Achim Jörres, Janusz Witowski

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Much has been learned about the mechanisms underlying cellular senescence. The pathways leading to senescence appear to vary, depending on the cell type and cell culture conditions. In this respect, little is known about senescence of human peritoneal mesothelial cells (HPMC). Previous studies have significantly differed in the reported proliferative lifespan of HPMC. Therefore, in the present study, we have examined how HPMC enter state of senescence under conditions typically used for HPMC culture. HPMC were isolated from omentum and grown into senescence. The cultures were assessed for the growth rate, the presence of senescence markers, activation of cell-cycle inhibitors, and the oxidative stress. HPMC were found to reach, on average, six population doublings before senescence. The terminal growth arrest was associated with decreased expression of Ki67 antigen, increased percentage of cells in the G1 phase, reduced early population doubling level cDNA-1 mRNA expression, and the presence of senescence-associated β-galactosidase. Compared with early-passage cells, the late-passage HPMC exhibited increased expression of p16INK4a but not of p21Cip1. In addition, these cells generated more reactive oxygen species and displayed increased presence of oxidatively modified DNA (8-hydroxy-2′-deoxyguanosine). These results demonstrate that early onset of senescence in omentum-derived HPMC may be associated with oxidative stress-induced upregulation of p16INK4a.

Original languageEnglish (US)
Pages (from-to)988-995
Number of pages8
JournalJournal of Applied Physiology
Volume100
Issue number3
DOIs
StatePublished - Mar 2006
Externally publishedYes

Fingerprint

Cell Culture Techniques
Omentum
Oxidative Stress
Galactosidases
Ki-67 Antigen
Cell Aging
G1 Phase
Growth
Population
Reactive Oxygen Species
Cell Cycle
Up-Regulation
Complementary DNA
Messenger RNA
DNA

Keywords

  • Cellular senescence
  • Oxidative stress

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Early loss of proliferative potential of human peritoneal mesothelial cells in culture : The role of p16INK4a-mediated premature senescence. / Ksia̧zek, Krzysztof; Piwocka, Katarzyna; Brzezińska, Agnieszka; Sikora, Ewa; Zabel, Maciej; Brȩborowicz, Andrzej; Jörres, Achim; Witowski, Janusz.

In: Journal of Applied Physiology, Vol. 100, No. 3, 03.2006, p. 988-995.

Research output: Contribution to journalArticle

Ksia̧zek, K, Piwocka, K, Brzezińska, A, Sikora, E, Zabel, M, Brȩborowicz, A, Jörres, A & Witowski, J 2006, 'Early loss of proliferative potential of human peritoneal mesothelial cells in culture: The role of p16INK4a-mediated premature senescence', Journal of Applied Physiology, vol. 100, no. 3, pp. 988-995. https://doi.org/10.1152/japplphysiol.01086.2005
Ksia̧zek, Krzysztof ; Piwocka, Katarzyna ; Brzezińska, Agnieszka ; Sikora, Ewa ; Zabel, Maciej ; Brȩborowicz, Andrzej ; Jörres, Achim ; Witowski, Janusz. / Early loss of proliferative potential of human peritoneal mesothelial cells in culture : The role of p16INK4a-mediated premature senescence. In: Journal of Applied Physiology. 2006 ; Vol. 100, No. 3. pp. 988-995.
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