Early events in guinea pig reticulocyte iron uptake

Simeon Pollack; Theresa Campana

doi:10.1016/0304-4165(81)90468-2

Early events in guinea pig reticulocyte iron uptake

Simeon Pollack, Theresa Campana

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Hemolysates, prepared from guinea pig reticulocytes incubated with ⁵⁹Fe-labelled serum, can be resolved into five peaks utilizing molecular sieve chromatography: ferritin, transferrin, hemoglobin, an M_r 17 000 fraction, and a low molecular weight fraction. The hemoglobin peak also contains a nonhemoglobin component (III-X), demonstrated by heme extraction and by isoelectric focusing. Transferrin, the III-X component and the low molecular weight fraction are the first to accumulate radioactive iron during the reticulocyte incubation. The ⁵⁹Fe in each of these also chases. Therefore, a role for these components as precursors to iron incorporation into heme is suggested.

Original language	English (US)
Pages (from-to)	366-373
Number of pages	8
Journal	BBA - General Subjects
Volume	673
Issue number	C
DOIs	https://doi.org/10.1016/0304-4165(81)90468-2
State	Published - 1981
Externally published	Yes

Keywords

(Reticulocyte)
Heme compound
Iron uptake

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0304-4165(81)90468-2

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title = "Early events in guinea pig reticulocyte iron uptake",

abstract = "Hemolysates, prepared from guinea pig reticulocytes incubated with 59Fe-labelled serum, can be resolved into five peaks utilizing molecular sieve chromatography: ferritin, transferrin, hemoglobin, an Mr 17 000 fraction, and a low molecular weight fraction. The hemoglobin peak also contains a nonhemoglobin component (III-X), demonstrated by heme extraction and by isoelectric focusing. Transferrin, the III-X component and the low molecular weight fraction are the first to accumulate radioactive iron during the reticulocyte incubation. The 59Fe in each of these also chases. Therefore, a role for these components as precursors to iron incorporation into heme is suggested.",

keywords = "(Reticulocyte), Heme compound, Iron uptake",

author = "Simeon Pollack and Theresa Campana",

note = "Funding Information: Supported by grants from the National Institutes of Health and the New York Community Trust.",

year = "1981",

doi = "10.1016/0304-4165(81)90468-2",

language = "English (US)",

volume = "673",

pages = "366--373",

journal = "Biochimica et Biophysica Acta - General Subjects",

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}

TY - JOUR

T1 - Early events in guinea pig reticulocyte iron uptake

AU - Pollack, Simeon

AU - Campana, Theresa

N1 - Funding Information: Supported by grants from the National Institutes of Health and the New York Community Trust.

PY - 1981

Y1 - 1981

N2 - Hemolysates, prepared from guinea pig reticulocytes incubated with 59Fe-labelled serum, can be resolved into five peaks utilizing molecular sieve chromatography: ferritin, transferrin, hemoglobin, an Mr 17 000 fraction, and a low molecular weight fraction. The hemoglobin peak also contains a nonhemoglobin component (III-X), demonstrated by heme extraction and by isoelectric focusing. Transferrin, the III-X component and the low molecular weight fraction are the first to accumulate radioactive iron during the reticulocyte incubation. The 59Fe in each of these also chases. Therefore, a role for these components as precursors to iron incorporation into heme is suggested.

AB - Hemolysates, prepared from guinea pig reticulocytes incubated with 59Fe-labelled serum, can be resolved into five peaks utilizing molecular sieve chromatography: ferritin, transferrin, hemoglobin, an Mr 17 000 fraction, and a low molecular weight fraction. The hemoglobin peak also contains a nonhemoglobin component (III-X), demonstrated by heme extraction and by isoelectric focusing. Transferrin, the III-X component and the low molecular weight fraction are the first to accumulate radioactive iron during the reticulocyte incubation. The 59Fe in each of these also chases. Therefore, a role for these components as precursors to iron incorporation into heme is suggested.

KW - (Reticulocyte)

KW - Heme compound

KW - Iron uptake

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DO - 10.1016/0304-4165(81)90468-2

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AN - SCOPUS:0019463270

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SP - 366

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JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

IS - C

ER -

Early events in guinea pig reticulocyte iron uptake

Abstract

Keywords

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