Early dystrophin disruption in the pathogenesis of experimental chronic Chagas cardiomyopathy

Cibele M. Prado, Mara R N Celes, Lygia M. Malvestio, Erica C. Campos, João S. Silva, Linda A. Jelicks, Herbert B. Tanowitz, Marcos A. Rossi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chronic Chagas cardiomyopathy evolves over a long period of time after initial infection by Trypanosoma cruzi. Similarly, a cardiomyopathy appears later in life in muscular dystrophies. This study tested the hypothesis that dystrophin levels are decreased in the early stage of T. cruzi-infected mice that precedes the later development of a cardiomyopathy. CD1 mice were infected with T. cruzi (Brazil strain), killed at 30 and 100 days post infection (dpi), and the intensity of inflammation, percentage of interstitial fibrosis, and dystrophin levels evaluated. Echocardiography and magnetic resonance imaging data were evaluated from 15 to 100 dpi. At 30 dpi an intense acute myocarditis with ruptured or intact intracellular parasite nests was observed. At 100 dpi a mild chronic fibrosing myocarditis was detected without parasites in the myocardium. Dystrophin was focally reduced or completely lost in cardiomyocytes at 30 dpi, with the reduction maintained up to 100 dpi. Concurrently, ejection fraction was reduced and the right ventricle was dilated. These findings support the hypothesis that the initial parasitic infection-induced myocardial dystrophin reduction/loss, maintained over time, might be essential to the late development of a cardiomyopathy in mice.

Original languageEnglish (US)
Pages (from-to)59-68
Number of pages10
JournalMicrobes and Infection
Volume14
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Chagas Cardiomyopathy
Dystrophin
Infection
Trypanosoma cruzi
Cardiomyopathies
Myocarditis
Parasites
Parasitic Diseases
Muscular Dystrophies
Cardiac Myocytes
Heart Ventricles
Brazil
Echocardiography
Myocardium
Fibrosis
Magnetic Resonance Imaging
Inflammation

Keywords

  • Chagas disease
  • Chronic Chagas cardiomyopathy
  • Dystrophin
  • Dystrophinopathy
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Prado, C. M., Celes, M. R. N., Malvestio, L. M., Campos, E. C., Silva, J. S., Jelicks, L. A., ... Rossi, M. A. (2012). Early dystrophin disruption in the pathogenesis of experimental chronic Chagas cardiomyopathy. Microbes and Infection, 14(1), 59-68. https://doi.org/10.1016/j.micinf.2011.08.010

Early dystrophin disruption in the pathogenesis of experimental chronic Chagas cardiomyopathy. / Prado, Cibele M.; Celes, Mara R N; Malvestio, Lygia M.; Campos, Erica C.; Silva, João S.; Jelicks, Linda A.; Tanowitz, Herbert B.; Rossi, Marcos A.

In: Microbes and Infection, Vol. 14, No. 1, 01.2012, p. 59-68.

Research output: Contribution to journalArticle

Prado, CM, Celes, MRN, Malvestio, LM, Campos, EC, Silva, JS, Jelicks, LA, Tanowitz, HB & Rossi, MA 2012, 'Early dystrophin disruption in the pathogenesis of experimental chronic Chagas cardiomyopathy', Microbes and Infection, vol. 14, no. 1, pp. 59-68. https://doi.org/10.1016/j.micinf.2011.08.010
Prado, Cibele M. ; Celes, Mara R N ; Malvestio, Lygia M. ; Campos, Erica C. ; Silva, João S. ; Jelicks, Linda A. ; Tanowitz, Herbert B. ; Rossi, Marcos A. / Early dystrophin disruption in the pathogenesis of experimental chronic Chagas cardiomyopathy. In: Microbes and Infection. 2012 ; Vol. 14, No. 1. pp. 59-68.
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