E2A-BP, A protein binding to E12/E47, inhibits differentiation of myoblasts

W. O. Endege, M. Jain, C. M. Hsieh, C. J. Kho, Nicholas E.S. Sibinga, M. Yoshizumi, C. Patterson, W. S. Lee, M. A. Perrella, M. A. Blanar, E. Haber, M. E. Lee

Research output: Contribution to journalArticle

Abstract

The E2A proteins E12 and E47 control cellular growth and differentiation. To identify potential partners that bind E2A proteins in vascular smooth muscle cells, we employed interaction cloning. Using the basic helix-loop-helix domain of E47 to screen for interacting proteins in a human aortic expression library, we isolated several cDNA clones. One clone, termed E2A-BP, was expressed preferentially in aortic smooth muscle cells in adult rats. The E2A-BP message was detected in fetal but not in adult skeletal muscle. Although E2A-BP is a nuclear protein lacking a helix-loop-helix domain, it bound selectively to El 2 and E47 but not MyoD and Id3 in an in vitro binding assay. By gel-shift analysis, E2A-BP inhibited the binding of E47 homodimers and E47-MyoD heterodimers to an E-box probe. In C2C12 myoblasts, overexpression of E2A-BP prevented the expression of muscle specific genes and the formation of myotubes induced by differentiauon medium. In contrast, expression of E2A-BP antisense transcripts in this cell type under conditions in which endogenous message was not expressed accelerated the formation of myotubes. Together these data indicate that, by regulating the function of E2A proteins, E2A-BP may have an important role in promoting cell growth and inhibiting terminal differentiation.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996
Externally publishedYes

Fingerprint

myoblasts
Myoblasts
protein binding
Protein Binding
Muscle
Helix-Loop-Helix Motifs
Skeletal Muscle Fibers
smooth muscle
myocytes
Smooth Muscle Myocytes
Proteins
proteins
Clone Cells
Cells
clones
Cloning
nuclear proteins
Cell growth
Electrophoretic Mobility Shift Assay
Growth

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Endege, W. O., Jain, M., Hsieh, C. M., Kho, C. J., Sibinga, N. E. S., Yoshizumi, M., ... Lee, M. E. (1996). E2A-BP, A protein binding to E12/E47, inhibits differentiation of myoblasts. FASEB Journal, 10(6).

E2A-BP, A protein binding to E12/E47, inhibits differentiation of myoblasts. / Endege, W. O.; Jain, M.; Hsieh, C. M.; Kho, C. J.; Sibinga, Nicholas E.S.; Yoshizumi, M.; Patterson, C.; Lee, W. S.; Perrella, M. A.; Blanar, M. A.; Haber, E.; Lee, M. E.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Endege, WO, Jain, M, Hsieh, CM, Kho, CJ, Sibinga, NES, Yoshizumi, M, Patterson, C, Lee, WS, Perrella, MA, Blanar, MA, Haber, E & Lee, ME 1996, 'E2A-BP, A protein binding to E12/E47, inhibits differentiation of myoblasts', FASEB Journal, vol. 10, no. 6.
Endege WO, Jain M, Hsieh CM, Kho CJ, Sibinga NES, Yoshizumi M et al. E2A-BP, A protein binding to E12/E47, inhibits differentiation of myoblasts. FASEB Journal. 1996;10(6).
Endege, W. O. ; Jain, M. ; Hsieh, C. M. ; Kho, C. J. ; Sibinga, Nicholas E.S. ; Yoshizumi, M. ; Patterson, C. ; Lee, W. S. ; Perrella, M. A. ; Blanar, M. A. ; Haber, E. ; Lee, M. E. / E2A-BP, A protein binding to E12/E47, inhibits differentiation of myoblasts. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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AU - Jain, M.

AU - Hsieh, C. M.

AU - Kho, C. J.

AU - Sibinga, Nicholas E.S.

AU - Yoshizumi, M.

AU - Patterson, C.

AU - Lee, W. S.

AU - Perrella, M. A.

AU - Blanar, M. A.

AU - Haber, E.

AU - Lee, M. E.

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