Dysfunctional endothelial nitric oxide biosynthesis in healthy smokers with impaired endothelium-dependent vasodilatation

Rajat S. Barua, John A. Ambrose, Lesley Jane Eales-Reynolds, Mary C. DeVoe, John G. Zervas, Dhanonjoy C. Saha

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

Background - The mechanisms involved in the dysfunction of both endothelium-dependent vasodilatation (EDV) and NO biosynthesis related to smoking are unclear. In this study, EDV was assessed in healthy smokers and nonsmokers in vivo and, using serum from the same individuals, was related to the NO biosynthetic pathway in vitro. Methods and Results - Flow-mediated EDV of the brachial artery was measured in 23 male patients (8 nonsmokers and 15 smokers). Serum was collected, added to confluent (≈85%) monolayers of human umbilical vein endothelial cells (HUVECs), and incubated for 12 hours. Basal and substance P-stimulated NO production was measured. The HUVECs used for measuring basal NO production were lysed, and both endothelial NO synthase (eNOS) protein expression and eNOS activity were determined. EDV was lower in smokers compared with nonsmokers (P

Original languageEnglish (US)
Pages (from-to)1905-1910
Number of pages6
JournalCirculation
Volume104
Issue number16
StatePublished - Oct 16 2001
Externally publishedYes

Fingerprint

Vasodilation
Endothelium
Nitric Oxide
Human Umbilical Vein Endothelial Cells
Nitric Oxide Synthase
Brachial Artery
Biosynthetic Pathways
Substance P
Serum
Smoking
Proteins

Keywords

  • Endothelium
  • Nitric oxide
  • Nitric oxide synthase
  • Smoking

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Barua, R. S., Ambrose, J. A., Eales-Reynolds, L. J., DeVoe, M. C., Zervas, J. G., & Saha, D. C. (2001). Dysfunctional endothelial nitric oxide biosynthesis in healthy smokers with impaired endothelium-dependent vasodilatation. Circulation, 104(16), 1905-1910.

Dysfunctional endothelial nitric oxide biosynthesis in healthy smokers with impaired endothelium-dependent vasodilatation. / Barua, Rajat S.; Ambrose, John A.; Eales-Reynolds, Lesley Jane; DeVoe, Mary C.; Zervas, John G.; Saha, Dhanonjoy C.

In: Circulation, Vol. 104, No. 16, 16.10.2001, p. 1905-1910.

Research output: Contribution to journalArticle

Barua, RS, Ambrose, JA, Eales-Reynolds, LJ, DeVoe, MC, Zervas, JG & Saha, DC 2001, 'Dysfunctional endothelial nitric oxide biosynthesis in healthy smokers with impaired endothelium-dependent vasodilatation', Circulation, vol. 104, no. 16, pp. 1905-1910.
Barua RS, Ambrose JA, Eales-Reynolds LJ, DeVoe MC, Zervas JG, Saha DC. Dysfunctional endothelial nitric oxide biosynthesis in healthy smokers with impaired endothelium-dependent vasodilatation. Circulation. 2001 Oct 16;104(16):1905-1910.
Barua, Rajat S. ; Ambrose, John A. ; Eales-Reynolds, Lesley Jane ; DeVoe, Mary C. ; Zervas, John G. ; Saha, Dhanonjoy C. / Dysfunctional endothelial nitric oxide biosynthesis in healthy smokers with impaired endothelium-dependent vasodilatation. In: Circulation. 2001 ; Vol. 104, No. 16. pp. 1905-1910.
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