Dynamics of single mRNPs in nuclei of living cells

Yaron Shav-Tal, Xavier Darzacq, Shailesh M. Shenoy, Dahlene Fusco, Susan M. Janicki, David L. Spector, Robert H. Singer

Research output: Contribution to journalArticle

362 Citations (Scopus)

Abstract

Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA) expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.

Original languageEnglish (US)
Pages (from-to)1797-1800
Number of pages4
JournalScience
Volume304
Issue number5678
DOIs
StatePublished - Jun 18 2004

Fingerprint

Cell Nucleus
Messenger RNA
Proteins
Chromatin
Protein Transport
Nuclear Proteins
Adenosine Triphosphate
Gene Expression

ASJC Scopus subject areas

  • General

Cite this

Shav-Tal, Y., Darzacq, X., Shenoy, S. M., Fusco, D., Janicki, S. M., Spector, D. L., & Singer, R. H. (2004). Dynamics of single mRNPs in nuclei of living cells. Science, 304(5678), 1797-1800. https://doi.org/10.1126/science.1099754

Dynamics of single mRNPs in nuclei of living cells. / Shav-Tal, Yaron; Darzacq, Xavier; Shenoy, Shailesh M.; Fusco, Dahlene; Janicki, Susan M.; Spector, David L.; Singer, Robert H.

In: Science, Vol. 304, No. 5678, 18.06.2004, p. 1797-1800.

Research output: Contribution to journalArticle

Shav-Tal, Y, Darzacq, X, Shenoy, SM, Fusco, D, Janicki, SM, Spector, DL & Singer, RH 2004, 'Dynamics of single mRNPs in nuclei of living cells', Science, vol. 304, no. 5678, pp. 1797-1800. https://doi.org/10.1126/science.1099754
Shav-Tal Y, Darzacq X, Shenoy SM, Fusco D, Janicki SM, Spector DL et al. Dynamics of single mRNPs in nuclei of living cells. Science. 2004 Jun 18;304(5678):1797-1800. https://doi.org/10.1126/science.1099754
Shav-Tal, Yaron ; Darzacq, Xavier ; Shenoy, Shailesh M. ; Fusco, Dahlene ; Janicki, Susan M. ; Spector, David L. ; Singer, Robert H. / Dynamics of single mRNPs in nuclei of living cells. In: Science. 2004 ; Vol. 304, No. 5678. pp. 1797-1800.
@article{b2e02e8c821f48e3b0b233a61dd9732f,
title = "Dynamics of single mRNPs in nuclei of living cells",
abstract = "Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA) expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.",
author = "Yaron Shav-Tal and Xavier Darzacq and Shenoy, {Shailesh M.} and Dahlene Fusco and Janicki, {Susan M.} and Spector, {David L.} and Singer, {Robert H.}",
year = "2004",
month = "6",
day = "18",
doi = "10.1126/science.1099754",
language = "English (US)",
volume = "304",
pages = "1797--1800",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5678",

}

TY - JOUR

T1 - Dynamics of single mRNPs in nuclei of living cells

AU - Shav-Tal, Yaron

AU - Darzacq, Xavier

AU - Shenoy, Shailesh M.

AU - Fusco, Dahlene

AU - Janicki, Susan M.

AU - Spector, David L.

AU - Singer, Robert H.

PY - 2004/6/18

Y1 - 2004/6/18

N2 - Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA) expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.

AB - Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA) expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.

UR - http://www.scopus.com/inward/record.url?scp=2942733310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942733310&partnerID=8YFLogxK

U2 - 10.1126/science.1099754

DO - 10.1126/science.1099754

M3 - Article

VL - 304

SP - 1797

EP - 1800

JO - Science

JF - Science

SN - 0036-8075

IS - 5678

ER -