Dynamics of an F-actin aggresome generated by the actin-stabilizing toxin jasplakinolide

Francisco Lazaro-Dieguez, Carmen Aguado, Eugenia Mato, Yován Sánchez-Ruíz, Inmaculada Esteban, Jordi Alberch, Erwin Knecht, Gustavo Egea

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In this study, we report the formation of several cytoplasmic inclusion bodies composed of filamentous actin (F-actin) and generated by experimental treatments using depolymerizing or stabilizing actin toxins in neuronal and non-neuronal mammalian cell lines. The actin-stabilizing toxin jasplakinolide (Jpk) induced, in a microtubule-dependent manner, a single, large F-actin aggregate, which contained β- and γ-actin, ADF/cofilin, cortactin, and the actin nucleator Arp2/3. This aggregate was tightly associated with the Golgi complex and mitochondria, and was surrounded by vimentin intermediate filaments, microtubules and MAP4. Therefore, the Jpk-induced single, large F-actin aggregate fits the established criteria for being considered an aggresome. Lysosomes and/or autophagic vacuoles, proteasomes and microtubules were found to directly participate in the dissolution of this F-actin aggresome. Finally, the model reported here is simple, highly reproducible and reversible, and it provides an opportunity to test pharmacological agents that interfere with the formation, maintenance and/or disappearance of F-actin-enriched pathological inclusion bodies.

Original languageEnglish (US)
Pages (from-to)1415-1425
Number of pages11
JournalJournal of Cell Science
Volume121
Issue number9
DOIs
StatePublished - May 1 2008
Externally publishedYes

Fingerprint

jasplakinolide
Actins
Inclusion Bodies
Microtubules
Cortactin
Actin Depolymerizing Factors
Intermediate Filaments

Keywords

  • Actin
  • Aggresome
  • Autophagy
  • Cytoskeleton
  • Huntingtin
  • Inclusion bodies
  • Jasplakinolide
  • Microtubules
  • Proteasome

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lazaro-Dieguez, F., Aguado, C., Mato, E., Sánchez-Ruíz, Y., Esteban, I., Alberch, J., ... Egea, G. (2008). Dynamics of an F-actin aggresome generated by the actin-stabilizing toxin jasplakinolide. Journal of Cell Science, 121(9), 1415-1425. https://doi.org/10.1242/jcs.017665

Dynamics of an F-actin aggresome generated by the actin-stabilizing toxin jasplakinolide. / Lazaro-Dieguez, Francisco; Aguado, Carmen; Mato, Eugenia; Sánchez-Ruíz, Yován; Esteban, Inmaculada; Alberch, Jordi; Knecht, Erwin; Egea, Gustavo.

In: Journal of Cell Science, Vol. 121, No. 9, 01.05.2008, p. 1415-1425.

Research output: Contribution to journalArticle

Lazaro-Dieguez, F, Aguado, C, Mato, E, Sánchez-Ruíz, Y, Esteban, I, Alberch, J, Knecht, E & Egea, G 2008, 'Dynamics of an F-actin aggresome generated by the actin-stabilizing toxin jasplakinolide', Journal of Cell Science, vol. 121, no. 9, pp. 1415-1425. https://doi.org/10.1242/jcs.017665
Lazaro-Dieguez, Francisco ; Aguado, Carmen ; Mato, Eugenia ; Sánchez-Ruíz, Yován ; Esteban, Inmaculada ; Alberch, Jordi ; Knecht, Erwin ; Egea, Gustavo. / Dynamics of an F-actin aggresome generated by the actin-stabilizing toxin jasplakinolide. In: Journal of Cell Science. 2008 ; Vol. 121, No. 9. pp. 1415-1425.
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