Dynamic resolution of functionally related gene sets in response to acute heat stress

Joseph D. Szustakowski, Penelope A. Kosinski, Christine A. Marrese, Jee Hyung Lee, Stephen J. Elliman, Nanguneri Nirmala, Daniel M. Kemp

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways. Results: Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours. Conclusion: In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.

Original languageEnglish (US)
Article number46
JournalBMC Molecular Biology
Volume8
DOIs
StatePublished - Jun 5 2007
Externally publishedYes

Fingerprint

Hot Temperature
Genes
Heat-Shock Response
Protein Folding
RNA Precursors
Skeletal Muscle Fibers
Proteasome Endopeptidase Complex
Computational Biology
Transcriptome
Proteolysis
Cluster Analysis
Protein folding
Shock
Bioinformatics
Microarrays
Transient analysis
Messenger RNA
Proteins
Processing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Szustakowski, J. D., Kosinski, P. A., Marrese, C. A., Lee, J. H., Elliman, S. J., Nirmala, N., & Kemp, D. M. (2007). Dynamic resolution of functionally related gene sets in response to acute heat stress. BMC Molecular Biology, 8, [46]. https://doi.org/10.1186/1471-2199-8-46

Dynamic resolution of functionally related gene sets in response to acute heat stress. / Szustakowski, Joseph D.; Kosinski, Penelope A.; Marrese, Christine A.; Lee, Jee Hyung; Elliman, Stephen J.; Nirmala, Nanguneri; Kemp, Daniel M.

In: BMC Molecular Biology, Vol. 8, 46, 05.06.2007.

Research output: Contribution to journalArticle

Szustakowski, JD, Kosinski, PA, Marrese, CA, Lee, JH, Elliman, SJ, Nirmala, N & Kemp, DM 2007, 'Dynamic resolution of functionally related gene sets in response to acute heat stress', BMC Molecular Biology, vol. 8, 46. https://doi.org/10.1186/1471-2199-8-46
Szustakowski JD, Kosinski PA, Marrese CA, Lee JH, Elliman SJ, Nirmala N et al. Dynamic resolution of functionally related gene sets in response to acute heat stress. BMC Molecular Biology. 2007 Jun 5;8. 46. https://doi.org/10.1186/1471-2199-8-46
Szustakowski, Joseph D. ; Kosinski, Penelope A. ; Marrese, Christine A. ; Lee, Jee Hyung ; Elliman, Stephen J. ; Nirmala, Nanguneri ; Kemp, Daniel M. / Dynamic resolution of functionally related gene sets in response to acute heat stress. In: BMC Molecular Biology. 2007 ; Vol. 8.
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