TY - JOUR
T1 - Dynamic resolution of functionally related gene sets in response to acute heat stress
AU - Szustakowski, Joseph D.
AU - Kosinski, Penelope A.
AU - Marrese, Christine A.
AU - Lee, Jee Hyung
AU - Elliman, Stephen J.
AU - Nirmala, Nanguneri
AU - Kemp, Daniel M.
PY - 2007/6/5
Y1 - 2007/6/5
N2 - Background: Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways. Results: Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours. Conclusion: In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.
AB - Background: Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways. Results: Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours. Conclusion: In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.
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U2 - 10.1186/1471-2199-8-46
DO - 10.1186/1471-2199-8-46
M3 - Article
C2 - 17550601
AN - SCOPUS:34347382715
SN - 1471-2199
VL - 8
JO - BMC Molecular Biology
JF - BMC Molecular Biology
M1 - 46
ER -