Dynamic resolution of functionally related gene sets in response to acute heat stress

Joseph D. Szustakowski, Penelope A. Kosinski, Christine A. Marrese, Jee Hyung Lee, Stephen J. Elliman, Nanguneri Nirmala, Daniel M. Kemp

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Background: Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways. Results: Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours. Conclusion: In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.

Original languageEnglish (US)
Article number46
JournalBMC Molecular Biology
StatePublished - Jun 5 2007
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology


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