Abstract
MCL-1 is a BCL-2 family protein implicated in the development and chemoresistance of human cancer. Unlike its anti-apoptotic homologs, Mcl-1 deletion has profound physiologic consequences, indicative of a broader role in homeostasis. We report that the BCL-2 homology 3 (BH3) α helix of MCL-1 can directly engage very long-chain acyl-CoA dehydrogenase (VLCAD), a key enzyme of the mitochondrial fatty acid β-oxidation (FAO) pathway. Proteomic analysis confirmed that the mitochondrial matrix isoform of MCL-1 (MCL-1Matrix) interacts with VLCAD. Mcl-1 deletion, or eliminating MCL-1Matrix alone, selectively deregulated long-chain FAO, causing increased flux through the pathway in response to nutrient deprivation. Transient elevation in MCL-1 upon serum withdrawal, a striking increase in MCL-1 BH3/VLCAD interaction upon palmitic acid titration, and direct modulation of enzymatic activity by the MCL-1 BH3 α helix are consistent with dynamic regulation. Thus, the MCL-1 BH3 interaction with VLCAD revealed a separable, gain-of-function role for MCL-1 in the regulation of lipid metabolism. MCL-1 is a formidable BCL-2 family protein implicated in human cancer and its genetic deletion causes profound physiologic consequences not compensated for by anti-apoptotic homologs. Escudero et al. report that the MCL-1 BH3 α helix directly binds to and modulates VLCAD, revealing a new role for MCL-1 in regulating lipid metabolism.
Original language | English (US) |
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Pages (from-to) | 729-743.e7 |
Journal | Molecular Cell |
Volume | 69 |
Issue number | 5 |
DOIs | |
State | Published - Mar 1 2018 |
Keywords
- BCL-2 family
- MCL-1
- VLCAD
- apoptosis
- fatty acid metabolism
- mitochondria
- mitochondrial matrix
- stapled peptide
- α helix
- β-oxidation
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology