Dynamic changes of histone H3 marks during Caenorhabditis elegans lifecycle revealed by middle-down proteomics

Simone Sidoli, Julien Vandamme, Anna Elisabetta Salcini, Ole N. Jensen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We applied a middle-down proteomics strategy for large-scale protein analysis during in vivo development of Caenorhabditis elegans. We characterized PTMs on histone H3 N-terminal tails at eight time points during the C. elegans lifecycle, including embryo, larval stages (L1-L4), dauer, and L1/L4 postdauer. Histones were analyzed by our optimized middle-down protein sequencing platform using high mass accuracy MS/MS. This allows quantification of intact histone tails and detailed characterization of distinct histone tails carrying cooccurring PTMs. We measured temporally distinct combinatorial PTM profiles during C. elegans development. We show that the doubly modified form H3K23me3K27me3, which is rare or nonexistent in mammals, is the most abundant PTM in all stages of C. elegans lifecycle. The abundance of H3K23me3 increased during development and it was mutually exclusive of the active marks H3K18ac, R26me1, and R40me1, suggesting a role for H3K23me3 in silent chromatin. We observed distinct PTM profiles for normal L1 larvae and for L1-postdauer larvae, or L4 and L4 postdauer, suggesting that histone PTMs mediate an epigenetic memory that is transmitted during dauer formation. Collectively, our data describe the dynamics of histone H3 combinatorial code during C. elegans lifecycle and demonstrate the feasibility of using middle-down proteomics to study in vivo development of multicellular organisms. All MS data have been deposited in the ProteomeXchange with identifier PXD002525 (http://proteomecentral.proteomexchange.org/dataset/PXD002525).

Original languageEnglish (US)
Pages (from-to)459-464
Number of pages6
JournalProteomics
Volume16
Issue number3
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Fingerprint

Histone Code
Pulse time modulation
Caenorhabditis elegans
Proteomics
Histones
Tail
Larva
Mammals
Protein Sequence Analysis
Epigenomics
Chromatin
Proteins
Embryonic Structures
Data storage equipment

Keywords

  • Animal proteomics
  • C. elegans
  • Histones
  • Lifecycle
  • Middle-down proteomics
  • Posttranslational modifications

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Dynamic changes of histone H3 marks during Caenorhabditis elegans lifecycle revealed by middle-down proteomics. / Sidoli, Simone; Vandamme, Julien; Salcini, Anna Elisabetta; Jensen, Ole N.

In: Proteomics, Vol. 16, No. 3, 01.02.2016, p. 459-464.

Research output: Contribution to journalArticle

Sidoli, Simone ; Vandamme, Julien ; Salcini, Anna Elisabetta ; Jensen, Ole N. / Dynamic changes of histone H3 marks during Caenorhabditis elegans lifecycle revealed by middle-down proteomics. In: Proteomics. 2016 ; Vol. 16, No. 3. pp. 459-464.
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