Dynamic actin structures stabilized by profilin

Toren Finkel, Julie A. Theriot, Kirk R. Dise, Gordon F. Tomaselli, Pascal J. Goldschmidt-Clermont

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Abstract

We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.

Original languageEnglish (US)
Pages (from-to)1510-1514
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number4
DOIs
Publication statusPublished - Feb 15 1994
Externally publishedYes

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Keywords

  • actin cytoskeleton
  • nucleotide exchange
  • overexpression

ASJC Scopus subject areas

  • General

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