Dynamic actin structures stabilized by profilin

Toren Finkel, Julie A. Theriot, Kirk R. Dise, Gordon F. Tomaselli, Pascal J. Goldschmidt-Clermont

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.

Original languageEnglish (US)
Pages (from-to)1510-1514
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number4
DOIs
StatePublished - Feb 15 1994
Externally publishedYes

Fingerprint

Profilins
Actins
Actin Cytoskeleton
Microfilament Proteins
Half-Life
Cell Line

Keywords

  • actin cytoskeleton
  • nucleotide exchange
  • overexpression

ASJC Scopus subject areas

  • General

Cite this

Dynamic actin structures stabilized by profilin. / Finkel, Toren; Theriot, Julie A.; Dise, Kirk R.; Tomaselli, Gordon F.; Goldschmidt-Clermont, Pascal J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 4, 15.02.1994, p. 1510-1514.

Research output: Contribution to journalArticle

Finkel, Toren ; Theriot, Julie A. ; Dise, Kirk R. ; Tomaselli, Gordon F. ; Goldschmidt-Clermont, Pascal J. / Dynamic actin structures stabilized by profilin. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 4. pp. 1510-1514.
@article{46a59655a94d442aa88cf7e304470a95,
title = "Dynamic actin structures stabilized by profilin",
abstract = "We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.",
keywords = "actin cytoskeleton, nucleotide exchange, overexpression",
author = "Toren Finkel and Theriot, {Julie A.} and Dise, {Kirk R.} and Tomaselli, {Gordon F.} and Goldschmidt-Clermont, {Pascal J.}",
year = "1994",
month = "2",
day = "15",
doi = "10.1073/pnas.91.4.1510",
language = "English (US)",
volume = "91",
pages = "1510--1514",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "4",

}

TY - JOUR

T1 - Dynamic actin structures stabilized by profilin

AU - Finkel, Toren

AU - Theriot, Julie A.

AU - Dise, Kirk R.

AU - Tomaselli, Gordon F.

AU - Goldschmidt-Clermont, Pascal J.

PY - 1994/2/15

Y1 - 1994/2/15

N2 - We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.

AB - We describe the production and analysis of clonal cell lines in which we have overexpressed human profilin, a small ubiquitous actin monomer binding protein, to assess the role of profilin on actin function in vivo. The concentration of filamentous actin is increased in cells with higher profilin levels, and actin filament half-life measured in these cells is directly proportional to the steady-state profilin concentration. The distribution of actin filaments is altered by profilin overexpression. While parallel actin bundles crossing the cells are virtually absent in cells overexpressing profilin, the submembranous actin network of these cells is denser than in control cells. These results suggest that in vivo profilin regulates the stability, and thereby distribution, of specific dynamic actin structures.

KW - actin cytoskeleton

KW - nucleotide exchange

KW - overexpression

UR - http://www.scopus.com/inward/record.url?scp=0028181252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028181252&partnerID=8YFLogxK

U2 - 10.1073/pnas.91.4.1510

DO - 10.1073/pnas.91.4.1510

M3 - Article

VL - 91

SP - 1510

EP - 1514

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 4

ER -