Dye-enhanced tissue welding using fibrinogen and continuous-wave argon lasers

S. K. Libutti, M. C. Oz, Roy S. Chuck, M. R. Treat, R. Nowygrod

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Despite their theoretical advantages, laser-welded anastomoses initially appear prone to rupture. Furthermore, the repair of defects seen in the weld following laser energy exposure is often hindered by thermal injury to the tissue edges. In this study, the immediate strength of these welds has been increased by the application of fibrinogen combined with a laser-energy-absorbing dye, fluorescein isothiocyanate (FITC, maximum absorbance 490 nm), to the anastomotic site prior to continuous-wave argon laser exposure (488-514 nm, 5.66 watts/cm2). A rabbit abdominal aortotomy model was employed. Data obtained immediately after welding demonstrated that the mean bursting pressure of welds created without fibrinogen (184 ± 65 mm Hg, n = 12) was significantly less than that of repairs with fibrinogen (407 ± 72 mm Hg, n = 11) (p<.001). Defects seen in the weld immediately after laser exposure could be easily repaired by additional application of fibrinogen and repeated laser treatment. Histologically, aortotomies repaired with fibrinogen demonstrate less thermal injury to host tissues than nonsolder repairs do. Laser soldering with exogenous fibrinogen significantly improves the bursting strength of primary laser-welded anastomoses and mitigates technical difficulties with laser tissue welding.

Original languageEnglish (US)
Pages (from-to)671-676
Number of pages6
JournalVascular Surgery
Volume24
Issue number9
StatePublished - 1990
Externally publishedYes

Fingerprint

Welding
Argon
Fibrinogen
Lasers
Coloring Agents
Hot Temperature
Fluorescein-5-isothiocyanate
Wounds and Injuries
Fluorescein
Rupture
Rabbits
Pressure

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Libutti, S. K., Oz, M. C., Chuck, R. S., Treat, M. R., & Nowygrod, R. (1990). Dye-enhanced tissue welding using fibrinogen and continuous-wave argon lasers. Vascular Surgery, 24(9), 671-676.

Dye-enhanced tissue welding using fibrinogen and continuous-wave argon lasers. / Libutti, S. K.; Oz, M. C.; Chuck, Roy S.; Treat, M. R.; Nowygrod, R.

In: Vascular Surgery, Vol. 24, No. 9, 1990, p. 671-676.

Research output: Contribution to journalArticle

Libutti, SK, Oz, MC, Chuck, RS, Treat, MR & Nowygrod, R 1990, 'Dye-enhanced tissue welding using fibrinogen and continuous-wave argon lasers', Vascular Surgery, vol. 24, no. 9, pp. 671-676.
Libutti, S. K. ; Oz, M. C. ; Chuck, Roy S. ; Treat, M. R. ; Nowygrod, R. / Dye-enhanced tissue welding using fibrinogen and continuous-wave argon lasers. In: Vascular Surgery. 1990 ; Vol. 24, No. 9. pp. 671-676.
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