During the early prediabetic period in NOD mice, the pathogenic CD8+ T-cell population comprises multiple antigenic specificities

Teresa P. DiLorenzo, Scott M. Lieberman, Toshiyuki Takaki, Shinichiro Honda, Harold D. Chapman, Pere Santamaria, David V. Serreze, Stanley G. Nathenson

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In the NOD mouse model of type 1 diabetes, major histocompatibility complex (MHC) class I-restricted CD8+ T cells are essential for disease development. However, the extent of diversity of their antigenic specificities during early pathogenesis remains unclear. An insulin-derived peptide was recently identified as the epitope for the NOD-derived diabetogenic T-cell clone G9C8. To explore the possibility that the early pathogenic CD8+ T-cell population comprises additional antigenic specificities, we employed the T-cell clones AI4 and NY8.3, both of which are pathogenic and represent specificities present in early insulitic lesions. The clones responded to distinct fractions of chromatographically separated class I MHC-bound peptides purified from NOD-derived NIT-1 β cells, and neither clone recognized the insulin-derived peptide. NIT-1 cells represent an unlimited peptide source that will allow for the future isolation and sequencing of the novel multiple epitopes targeted early in the autoimmune response by pathogenic CD8+ T cells.

Original languageEnglish (US)
Pages (from-to)332-341
Number of pages10
JournalClinical Immunology
Volume105
Issue number3
DOIs
StatePublished - Dec 1 2002

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Inbred NOD Mouse
Epitopes
T-Lymphocytes
Clone Cells
Peptides
Population
Major Histocompatibility Complex
Insulin
Autoimmunity
Type 1 Diabetes Mellitus

Keywords

  • Autoimmunity
  • CD8 T cells
  • Epitopes
  • NOD mice
  • Type 1 diabetes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

During the early prediabetic period in NOD mice, the pathogenic CD8+ T-cell population comprises multiple antigenic specificities. / DiLorenzo, Teresa P.; Lieberman, Scott M.; Takaki, Toshiyuki; Honda, Shinichiro; Chapman, Harold D.; Santamaria, Pere; Serreze, David V.; Nathenson, Stanley G.

In: Clinical Immunology, Vol. 105, No. 3, 01.12.2002, p. 332-341.

Research output: Contribution to journalArticle

DiLorenzo, TP, Lieberman, SM, Takaki, T, Honda, S, Chapman, HD, Santamaria, P, Serreze, DV & Nathenson, SG 2002, 'During the early prediabetic period in NOD mice, the pathogenic CD8+ T-cell population comprises multiple antigenic specificities', Clinical Immunology, vol. 105, no. 3, pp. 332-341. https://doi.org/10.1006/clim.2002.5298
DiLorenzo, Teresa P. ; Lieberman, Scott M. ; Takaki, Toshiyuki ; Honda, Shinichiro ; Chapman, Harold D. ; Santamaria, Pere ; Serreze, David V. ; Nathenson, Stanley G. / During the early prediabetic period in NOD mice, the pathogenic CD8+ T-cell population comprises multiple antigenic specificities. In: Clinical Immunology. 2002 ; Vol. 105, No. 3. pp. 332-341.
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