Duplication, gene conversion, and genetic diversity in the species-specific acyl-CoA synthetase gene family of Plasmodium falciparum

Lara L. Bethke, Martine Zilversmit, Kaare Nielsen, Johanna P. Daily, Sarah K. Volkman, Daouda Ndiaye, Elena R. Lozovsky, Daniel L. Hartl, Dyann F. Wirth

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

While genes encoding antigens and other highly polymorphic proteins are commonly found in subtelomeres, it is unusual to find a small family of housekeeping genes in these regions. We found that in the species Plasmodium falciparum only, a non-subtelomeric acyl-CoA synthetase (ACS) gene has expanded into a family of duplicated genes mainly located in the subtelomeres of the genome. We identified the putative parent of the duplicated family by analysis of synteny and phylogeny relative to other Plasmodium ACS genes. All ten ACS paralogs are transcribed in erythrocytic stages of laboratory and field isolates. We identified and confirmed a recent double gene conversion event involving ACS genes on three different chromosomes of isolate 3D7, resulting in the creation of a new hybrid gene. Southern hybridization analysis of geographically diverse P. falciparum isolates provides evidence for the strikingly global conservation of the ACS gene family, but also for some chromosomal events, including deletion and recombination, involving the duplicated paralogs. We found a dramatically higher rate of non-synonymous substitutions per non-synonymous site than synonymous substitutions per synonymous site in the closely related ACS paralogs we sequenced, suggesting that these genes are under a form of selection that favors change in the state of the protein. We also found that the gene encoding acyl-CoA binding protein has expanded and diversified in P. falciparum. We have described a new class of subtelomeric gene family with a unique capacity for diversity in P. falciparum.

Original languageEnglish (US)
Pages (from-to)10-24
Number of pages15
JournalMolecular and Biochemical Parasitology
Volume150
Issue number1
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

Coenzyme A Ligases
Gene Conversion
Plasmodium falciparum
Genes
Diazepam Binding Inhibitor
Synteny
Plasmodium
Essential Genes
Phylogeny
Genetic Recombination
Proteins
Chromosomes
Genome

Keywords

  • Acyl-CoA synthetase
  • Fatty acid metabolism
  • Gene conversion
  • Gene family
  • Malaria
  • Plasmodium falciparum
  • Subtelomere

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Duplication, gene conversion, and genetic diversity in the species-specific acyl-CoA synthetase gene family of Plasmodium falciparum. / Bethke, Lara L.; Zilversmit, Martine; Nielsen, Kaare; Daily, Johanna P.; Volkman, Sarah K.; Ndiaye, Daouda; Lozovsky, Elena R.; Hartl, Daniel L.; Wirth, Dyann F.

In: Molecular and Biochemical Parasitology, Vol. 150, No. 1, 11.2006, p. 10-24.

Research output: Contribution to journalArticle

Bethke, Lara L. ; Zilversmit, Martine ; Nielsen, Kaare ; Daily, Johanna P. ; Volkman, Sarah K. ; Ndiaye, Daouda ; Lozovsky, Elena R. ; Hartl, Daniel L. ; Wirth, Dyann F. / Duplication, gene conversion, and genetic diversity in the species-specific acyl-CoA synthetase gene family of Plasmodium falciparum. In: Molecular and Biochemical Parasitology. 2006 ; Vol. 150, No. 1. pp. 10-24.
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