Duchenne dystrophic muscle develops lesions in long-term coculture with mouse spinal cord

E. R. Peterson, E. B. Masurovsky, A. J. Spiro, S. M. Crain

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

When strips of human skeletal muscle from biopsies of normal children and donors with Duchenne muscular dystrophy (DMD) are explanted in organotypic coculture with fetal mouse spinal cord, many regenerating muscle fibers develop, become innervated, and maintain a remarkable degree of mature structure and function for more than 3-6 months in vitro. Sequential light microscopy in correlation with electron-microscopic and electrophysiologic analyses showed that despite cross-species innervation, these human muscle fibers develop stable cross-striations, peripherally positioned myonuclei, and mature, functional motor endplates. Of special interest is the onset of significant progressive abnormalities, e.g., unusual focal myofibrillar lesions, in substantial numbers of innervated mature DMD muscle fibers after 2-4 months in culture. The focal myofibrillar lesions were not detected in normal muscle fibers maintained as long as 6 months in coculture, nor are they comparable to the generalized loss of cross-striations observed in muscle atrophy following in vitro denervation of mature DMD fibers.

Original languageEnglish (US)
Pages (from-to)787-808
Number of pages22
JournalMuscle and Nerve
Volume9
Issue number9
StatePublished - 1986

Fingerprint

Coculture Techniques
Duchenne Muscular Dystrophy
Spinal Cord
Muscles
Motor Endplate
Muscular Atrophy
Denervation
Microscopy
Skeletal Muscle
Tissue Donors
Electrons
Biopsy
Light
In Vitro Techniques

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Peterson, E. R., Masurovsky, E. B., Spiro, A. J., & Crain, S. M. (1986). Duchenne dystrophic muscle develops lesions in long-term coculture with mouse spinal cord. Muscle and Nerve, 9(9), 787-808.

Duchenne dystrophic muscle develops lesions in long-term coculture with mouse spinal cord. / Peterson, E. R.; Masurovsky, E. B.; Spiro, A. J.; Crain, S. M.

In: Muscle and Nerve, Vol. 9, No. 9, 1986, p. 787-808.

Research output: Contribution to journalArticle

Peterson, ER, Masurovsky, EB, Spiro, AJ & Crain, SM 1986, 'Duchenne dystrophic muscle develops lesions in long-term coculture with mouse spinal cord', Muscle and Nerve, vol. 9, no. 9, pp. 787-808.
Peterson ER, Masurovsky EB, Spiro AJ, Crain SM. Duchenne dystrophic muscle develops lesions in long-term coculture with mouse spinal cord. Muscle and Nerve. 1986;9(9):787-808.
Peterson, E. R. ; Masurovsky, E. B. ; Spiro, A. J. ; Crain, S. M. / Duchenne dystrophic muscle develops lesions in long-term coculture with mouse spinal cord. In: Muscle and Nerve. 1986 ; Vol. 9, No. 9. pp. 787-808.
@article{534d06b141fd43b6a80e914cb5cdec47,
title = "Duchenne dystrophic muscle develops lesions in long-term coculture with mouse spinal cord",
abstract = "When strips of human skeletal muscle from biopsies of normal children and donors with Duchenne muscular dystrophy (DMD) are explanted in organotypic coculture with fetal mouse spinal cord, many regenerating muscle fibers develop, become innervated, and maintain a remarkable degree of mature structure and function for more than 3-6 months in vitro. Sequential light microscopy in correlation with electron-microscopic and electrophysiologic analyses showed that despite cross-species innervation, these human muscle fibers develop stable cross-striations, peripherally positioned myonuclei, and mature, functional motor endplates. Of special interest is the onset of significant progressive abnormalities, e.g., unusual focal myofibrillar lesions, in substantial numbers of innervated mature DMD muscle fibers after 2-4 months in culture. The focal myofibrillar lesions were not detected in normal muscle fibers maintained as long as 6 months in coculture, nor are they comparable to the generalized loss of cross-striations observed in muscle atrophy following in vitro denervation of mature DMD fibers.",
author = "Peterson, {E. R.} and Masurovsky, {E. B.} and Spiro, {A. J.} and Crain, {S. M.}",
year = "1986",
language = "English (US)",
volume = "9",
pages = "787--808",
journal = "Muscle and Nerve",
issn = "0148-639X",
publisher = "John Wiley and Sons Inc.",
number = "9",

}

TY - JOUR

T1 - Duchenne dystrophic muscle develops lesions in long-term coculture with mouse spinal cord

AU - Peterson, E. R.

AU - Masurovsky, E. B.

AU - Spiro, A. J.

AU - Crain, S. M.

PY - 1986

Y1 - 1986

N2 - When strips of human skeletal muscle from biopsies of normal children and donors with Duchenne muscular dystrophy (DMD) are explanted in organotypic coculture with fetal mouse spinal cord, many regenerating muscle fibers develop, become innervated, and maintain a remarkable degree of mature structure and function for more than 3-6 months in vitro. Sequential light microscopy in correlation with electron-microscopic and electrophysiologic analyses showed that despite cross-species innervation, these human muscle fibers develop stable cross-striations, peripherally positioned myonuclei, and mature, functional motor endplates. Of special interest is the onset of significant progressive abnormalities, e.g., unusual focal myofibrillar lesions, in substantial numbers of innervated mature DMD muscle fibers after 2-4 months in culture. The focal myofibrillar lesions were not detected in normal muscle fibers maintained as long as 6 months in coculture, nor are they comparable to the generalized loss of cross-striations observed in muscle atrophy following in vitro denervation of mature DMD fibers.

AB - When strips of human skeletal muscle from biopsies of normal children and donors with Duchenne muscular dystrophy (DMD) are explanted in organotypic coculture with fetal mouse spinal cord, many regenerating muscle fibers develop, become innervated, and maintain a remarkable degree of mature structure and function for more than 3-6 months in vitro. Sequential light microscopy in correlation with electron-microscopic and electrophysiologic analyses showed that despite cross-species innervation, these human muscle fibers develop stable cross-striations, peripherally positioned myonuclei, and mature, functional motor endplates. Of special interest is the onset of significant progressive abnormalities, e.g., unusual focal myofibrillar lesions, in substantial numbers of innervated mature DMD muscle fibers after 2-4 months in culture. The focal myofibrillar lesions were not detected in normal muscle fibers maintained as long as 6 months in coculture, nor are they comparable to the generalized loss of cross-striations observed in muscle atrophy following in vitro denervation of mature DMD fibers.

UR - http://www.scopus.com/inward/record.url?scp=0022502734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022502734&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 787

EP - 808

JO - Muscle and Nerve

JF - Muscle and Nerve

SN - 0148-639X

IS - 9

ER -