Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum

Menno L.W. Knetsch, Stefan J.P. Epskamp, Paul W. Schenk, Yiwen Wang, Jeffrey E. Segall, B. Ewa Snaar-Jagalska

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Dictyostelium discoideum expresses two Extracellular signal Regulated Kinases, ERK1 and ERK2, which are involved in growth, multicellular development and regulation of adenylyl cyclase. Binding of extracellular cAMP to cAMP receptor 1, a G-protein coupled cell surface receptor, transiently stimulates phosphorylation, activation and nuclear translocation of ERK2. Activation of ERK2 by cAMP is dependent on hetero-trimeric G-proteins, since activation of ERK2 is absent in cells lacking the Gα4 subunit. The small G-protein rasD also activates ERK2. In cells overexpressing a mutated, constitutively active rasD, ERK2 activity is elevated prior to cAMP stimulation. Intracellular cAMP and cAMP-dependent protein kinase (PKA) are essential for adaptation of the ERK2 response. This report shows that multiple signalling pathways are involved in regulation of ERK2 activity in D. discoideum.

Original languageEnglish (US)
Pages (from-to)3361-3368
Number of pages8
JournalEMBO Journal
Volume15
Issue number13
StatePublished - Jul 1 1996

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Keywords

  • Cross-talk
  • Dictyostelium
  • G-protein
  • MAP kinase
  • ras

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Knetsch, M. L. W., Epskamp, S. J. P., Schenk, P. W., Wang, Y., Segall, J. E., & Snaar-Jagalska, B. E. (1996). Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum. EMBO Journal, 15(13), 3361-3368.