Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum

Menno L W Knetsch, Stefan J P Epskamp, Paul W. Schenk, Yiwen Wang, Jeffrey E. Segall, B. Ewa Snaar-Jagalska

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Dictyostelium discoideum expresses two Extracellular signal Regulated Kinases, ERK1 and ERK2, which are involved in growth, multicellular development and regulation of adenylyl cyclase. Binding of extracellular cAMP to cAMP receptor 1, a G-protein coupled cell surface receptor, transiently stimulates phosphorylation, activation and nuclear translocation of ERK2. Activation of ERK2 by cAMP is dependent on hetero-trimeric G-proteins, since activation of ERK2 is absent in cells lacking the Gα4 subunit. The small G-protein rasD also activates ERK2. In cells overexpressing a mutated, constitutively active rasD, ERK2 activity is elevated prior to cAMP stimulation. Intracellular cAMP and cAMP-dependent protein kinase (PKA) are essential for adaptation of the ERK2 response. This report shows that multiple signalling pathways are involved in regulation of ERK2 activity in D. discoideum.

Original languageEnglish (US)
Pages (from-to)3361-3368
Number of pages8
JournalEMBO Journal
Volume15
Issue number13
StatePublished - Jul 1 1996

Fingerprint

Dictyostelium
GTP-Binding Proteins
Chemical activation
Cyclic AMP Receptors
Monomeric GTP-Binding Proteins
Extracellular Signal-Regulated MAP Kinases
Cell Surface Receptors
Cyclic AMP-Dependent Protein Kinases
Growth and Development
Adenylyl Cyclases
Phosphorylation

Keywords

  • Cross-talk
  • Dictyostelium
  • G-protein
  • MAP kinase
  • ras

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Knetsch, M. L. W., Epskamp, S. J. P., Schenk, P. W., Wang, Y., Segall, J. E., & Snaar-Jagalska, B. E. (1996). Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum. EMBO Journal, 15(13), 3361-3368.

Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum. / Knetsch, Menno L W; Epskamp, Stefan J P; Schenk, Paul W.; Wang, Yiwen; Segall, Jeffrey E.; Snaar-Jagalska, B. Ewa.

In: EMBO Journal, Vol. 15, No. 13, 01.07.1996, p. 3361-3368.

Research output: Contribution to journalArticle

Knetsch, MLW, Epskamp, SJP, Schenk, PW, Wang, Y, Segall, JE & Snaar-Jagalska, BE 1996, 'Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum', EMBO Journal, vol. 15, no. 13, pp. 3361-3368.
Knetsch MLW, Epskamp SJP, Schenk PW, Wang Y, Segall JE, Snaar-Jagalska BE. Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum. EMBO Journal. 1996 Jul 1;15(13):3361-3368.
Knetsch, Menno L W ; Epskamp, Stefan J P ; Schenk, Paul W. ; Wang, Yiwen ; Segall, Jeffrey E. ; Snaar-Jagalska, B. Ewa. / Dual role of cAMP and involvement of both G-proteins and ras in regulation of ERK2 in Dictyostelium discoideum. In: EMBO Journal. 1996 ; Vol. 15, No. 13. pp. 3361-3368.
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