Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration

Dong Zhang, Kyle D. Grode, Shannon F. Stewman, Juan Daniel Diaz-Valencia, Emily Liebling, Uttama Rath, Tania Riera, Joshua D. Currie, Daniel W. Buster, Ana B. Asenjo, Hernando J. Sosa, Jennifer L. Ross, Ao Ma, Stephen L. Rogers, David J. Sharp

Research output: Contribution to journalArticle

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Abstract

Regulation of microtubule dynamics at the cell cortex is important for cell motility, morphogenesis and division. Here we show that the Drosophila katanin Dm-Kat60 functions to generate a dynamic cortical-microtubule interface in interphase cells. Dm-Kat60 concentrates at the cell cortex of S2 Drosophila cells during interphase, where it suppresses the polymerization of microtubule plus-ends, thereby preventing the formation of aberrantly dense cortical arrays. Dm-Kat60 also localizes at the leading edge of migratory D17 Drosophila cells and negatively regulates multiple parameters of their motility. Finally, in vitro, Dm-Kat60 severs and depolymerizes microtubules from their ends. On the basis of these data, we propose that Dm-Kat60 removes tubulin from microtubule lattice or microtubule ends that contact specific cortical sites to prevent stable and/or lateral attachments. The asymmetric distribution of such an activity could help generate regional variations in microtubule behaviours involved in cell migration.

Original languageEnglish (US)
Pages (from-to)361-372
Number of pages12
JournalNature Cell Biology
Volume13
Issue number4
DOIs
StatePublished - Apr 2011

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Microtubules
Drosophila
Cell Movement
Interphase
Tubulin
katanin
Morphogenesis
Polymerization
Cell Division

ASJC Scopus subject areas

  • Cell Biology

Cite this

Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration. / Zhang, Dong; Grode, Kyle D.; Stewman, Shannon F.; Diaz-Valencia, Juan Daniel; Liebling, Emily; Rath, Uttama; Riera, Tania; Currie, Joshua D.; Buster, Daniel W.; Asenjo, Ana B.; Sosa, Hernando J.; Ross, Jennifer L.; Ma, Ao; Rogers, Stephen L.; Sharp, David J.

In: Nature Cell Biology, Vol. 13, No. 4, 04.2011, p. 361-372.

Research output: Contribution to journalArticle

Zhang, D, Grode, KD, Stewman, SF, Diaz-Valencia, JD, Liebling, E, Rath, U, Riera, T, Currie, JD, Buster, DW, Asenjo, AB, Sosa, HJ, Ross, JL, Ma, A, Rogers, SL & Sharp, DJ 2011, 'Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration', Nature Cell Biology, vol. 13, no. 4, pp. 361-372. https://doi.org/10.1038/ncb2206
Zhang, Dong ; Grode, Kyle D. ; Stewman, Shannon F. ; Diaz-Valencia, Juan Daniel ; Liebling, Emily ; Rath, Uttama ; Riera, Tania ; Currie, Joshua D. ; Buster, Daniel W. ; Asenjo, Ana B. ; Sosa, Hernando J. ; Ross, Jennifer L. ; Ma, Ao ; Rogers, Stephen L. ; Sharp, David J. / Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration. In: Nature Cell Biology. 2011 ; Vol. 13, No. 4. pp. 361-372.
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